Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration

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Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration

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Title: Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration
Author: Elewa, Ahmed; Wang, Heng; Talavera-López, Carlos; Joven, Alberto; Brito, Gonçalo; Kumar, Anoop; Hameed, L. Shahul; Penrad-Mobayed, May; Yao, Zeyu; Zamani, Neda; Abbas, Yamen; Abdullayev, Ilgar; Sandberg, Rickard; Grabherr, Manfred; Andersson, Björn; Simon, András

Note: Order does not necessarily reflect citation order of authors.

Citation: Elewa, A., H. Wang, C. Talavera-López, A. Joven, G. Brito, A. Kumar, L. S. Hameed, et al. 2017. “Reading and editing the Pleurodeles waltl genome reveals novel features of tetrapod regeneration.” Nature Communications 8 (1): 2286. doi:10.1038/s41467-017-01964-9. http://dx.doi.org/10.1038/s41467-017-01964-9.
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Abstract: Salamanders exhibit an extraordinary ability among vertebrates to regenerate complex body parts. However, scarce genomic resources have limited our understanding of regeneration in adult salamanders. Here, we present the ~20 Gb genome and transcriptome of the Iberian ribbed newt Pleurodeles waltl, a tractable species suitable for laboratory research. We find that embryonic stem cell-specific miRNAs mir-93b and mir-427/430/302, as well as Harbinger DNA transposons carrying the Myb-like proto-oncogene have expanded dramatically in the Pleurodeles waltl genome and are co-expressed during limb regeneration. Moreover, we find that a family of salamander methyltransferases is expressed specifically in adult appendages. Using CRISPR/Cas9 technology to perturb transcription factors, we demonstrate that, unlike the axolotl, Pax3 is present and necessary for development and that contrary to mammals, muscle regeneration is normal without functional Pax7 gene. Our data provide a foundation for comparative genomic studies that generate models for the uneven distribution of regenerative capacities among vertebrates.
Published Version: doi:10.1038/s41467-017-01964-9
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5741667/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34652068
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