Show simple item record

dc.contributor.authorEl Annan, Jaafar
dc.contributor.authorGoyal, Sunali
dc.contributor.authorZhang, Qiang
dc.contributor.authorFreeman, Gordon James
dc.contributor.authorSharpe, Arlene Helen
dc.contributor.authorDana, Reza
dc.date.accessioned2018-02-06T22:15:50Z
dc.date.issued2010
dc.identifierQuick submit: 2017-06-18T19:56:34-0400
dc.identifier.citationEl Annan, Jaafar, Sunali Goyal, Qiang Zhang, Gordon J. Freeman, Arlene H. Sharpe, and Reza Dana. 2010. “Regulation of T-Cell Chemotaxis by Programmed Death-Ligand 1 (PD-L1) in Dry Eye–Associated Corneal Inflammation.” Investigative Opthalmology & Visual Science 51 (7) (July 1): 3418. doi:10.1167/iovs.09-3684.en_US
dc.identifier.issn1552-5783en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34787802
dc.description.abstractPurpose. Given that dry eye disease (DED) is associated with T cell–mediated inflammation of the ocular surface and that PD-L1 is an important negative or inhibitory regulator of immune responses constitutively expressed at high levels by corneal epithelial cells, the authors studied the expression and function of PD-L1 in DED. Methods. Dry eye was induced in untreated wild-type mice, PD-L1−/− mice, and wild-type mice treated with anti–PD-L1 antibody by exposing these mice to a desiccating environment in the controlled environment chamber modified with subcutaneous administration of scopolamine. Real-time PCR was used to quantify the expression of chemokine gene transcript levels of multiple CC and CXC chemokine ligands and receptors. Epifluorescence microscopy was used to evaluate corneal infiltration of CD3+ T cells after immunohistochemical staining. Results. The increased expression of specific chemokine ligands and receptors in PD-L1−/− corneas of normal mice is associated with significant increases in T-cell homing into these corneas. Similar, and more enhanced, increases in T-cell infiltration were observed in PD-L1−/− DED mice or DED mice treated with anti–PD-L1 antibody compared with controls. In addition, the authors found significantly decreased expression of PD-L1 by corneal epithelial cells in DED and significantly increased corneal fluorescein staining score with PD-L1 functional blockade using anti–PD-L1 antibody. Conclusions. Downregulation of corneal epithelial PD-L1 amplifies dry eye–associated corneal inflammation and epitheliopathy by increasing the expression of chemokine ligands and receptors that promote T-cell homing to the ocular surface.en_US
dc.language.isoen_USen_US
dc.publisherAssociation for Research in Vision and Ophthalmology (ARVO)en_US
dc.relation.isversionof10.1167/iovs.09-3684en_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3979489/en_US
dash.licenseLAA
dc.titleRegulation of T-Cell Chemotaxis by Programmed Death-Ligand 1 (PD-L1) in Dry Eye–Associated Corneal Inflammationen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-18T23:56:36Z
dc.description.versionVersion of Recorden_US
dc.relation.journalInvestigative Opthalmology & Visual Scienceen_US
dash.depositing.authorDana, Reza
dc.date.available2010
dc.date.available2018-02-06T22:15:50Z
dc.identifier.doi10.1167/iovs.09-3684*
dash.contributor.affiliatedSharpe, Arlene
dash.contributor.affiliatedFreeman, Gordon
dash.contributor.affiliatedDana, Reza


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record