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dc.contributor.authorInomata, Takenori
dc.contributor.authorMashaghi, Alireza
dc.contributor.authorDi Zazzo, Antonio
dc.contributor.authorDana, Reza
dc.date.accessioned2018-02-20T21:38:41Z
dc.date.issued2015
dc.identifierQuick submit: 2017-06-18T21:47:48-0400
dc.identifier.citationInomata, Takenori, Alireza Mashaghi, Antonio Di Zazzo, and Reza Dana. 2015. “Ocular Surgical Models for Immune and Angiogenic Responses.” Journal of Biological Methods 2 (3) (September 21): 27. doi:10.14440/jbm.2015.78.en_US
dc.identifier.issn2326-9901en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34854267
dc.description.abstractCorneal transplantation serves as a reproducible and simple surgical model to study mechanisms regulating immunity and angiogenesis. The simplicity of the model allows for systematic analysis of different mechanisms involved in immune and angiogenic privilege and their failures. This protocol describes how to induce neovessels and inflammation in an actively regulated avascular and immune-privileged site. This involves placing intra-stromal corneal sutures for two weeks, disrupting the privileges, and performing corneal transplantation subsequently. Privileged and non-privileged recipient responses to donor cornea can be compared to identify key immunological mechanisms that underlie angiogenesis and graft rejection. This protocol can also be adapted to the growing repertoire of genetic models available in the mouse, and is a valuable tool to elucidate molecular mechanisms mediating acceptance or failure of corneal graft. The model could be used to assess the potential of therapeutic molecules to enhance graft survival in vivo.en_US
dc.language.isoen_USen_US
dc.publisherJournal of Biological Methodsen_US
dc.relation.isversionof10.14440/jbm.2015.78en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4636218/en_US
dash.licenseOAP
dc.titleOcular surgical models for immune and angiogenic responsesen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-19T01:47:53Z
dc.description.versionAccepted Manuscripten_US
dc.relation.journalJournal of Biological Methodsen_US
dash.depositing.authorDana, Reza
dc.date.available2015
dc.date.available2018-02-20T21:38:41Z
dc.identifier.doi10.14440/jbm.2015.78*
workflow.legacycommentscat.complete cfr.complete (clc)en_US
dash.contributor.affiliatedDana, Reza


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