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dc.contributor.authorCrnej, A.
dc.contributor.authorOmoto, M.
dc.contributor.authorDohlman, T. H.
dc.contributor.authorDohlman, Claes Henrik
dc.contributor.authorDana, Reza
dc.date.accessioned2018-02-20T21:44:23Z
dc.date.issued2014
dc.identifierQuick submit: 2017-06-18T21:30:40-0400
dc.identifier.citationCrnej, A., M. Omoto, T. H. Dohlman, C. H. Dohlman, and R. Dana. 2014. “Corneal Inflammation After Miniature Keratoprosthesis Implantation.” Investigative Ophthalmology & Visual Science 56 (1) (December 16): 185–189. doi:10.1167/iovs.14-15884.en_US
dc.identifier.issn0146-0404en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34854278
dc.description.abstractPurpose.: To compare corneal inflammation after syngeneic and allogeneic penetrating keratoplasty (PK) with miniature Keratoprosthesis (m-KPro) implantation in mice. Methods.: BALB/C (syngeneic) or C57BL/6 (allogeneic) corneas were transplanted onto BALB/C host beds as part of PK or m-KPro implantation. Corneal inflammation was assessed by determining the frequencies of CD45+ leukocytes, CD4+ T cells, CD11b+ cells, and Gr-1+ granulocytes/monocytes by flow cytometry at 2, 4, and 8 weeks post transplantation. In addition, expression levels of the proinflammatory cytokines TNF-α and IL-1β were analyzed using real-time qPCR at 8 weeks post transplantation. Results.: Cell frequencies in the syngeneic (syn) and allogeneic (allo) m-KPro groups were higher compared with the syngeneic and allogeneic PK groups, respectively, at all time points. However, after week 4, frequencies of all analyzed immune cells were higher in the alloPK group as compared with synKPro group. At 8 weeks, the expression of TNF-α was higher in synKPro, alloPK, and alloKPro groups compared with the naïve and synPK groups. The expression of IL-1β was significantly higher in both KPro groups as compared with PK groups. Conclusions.: Although the m-KPro device augments the inflammatory response in the cornea after its implantation, allogenicity (of the carrier tissue) is also a significant contributor to corneal inflammation. These data suggest that using syngeneic or decellularized corneal tissue as a Boston-KPro carrier could reduce the postoperative inflammation response.en_US
dc.language.isoen_USen_US
dc.publisherAssociation for Research in Vision and Ophthalmology (ARVO)en_US
dc.relation.isversionof10.1167/iovs.14-15884en_US
dash.licenseLAA
dc.subjectBoston Keratoprosthesisen_US
dc.subjectpenetrating keratoplastyen_US
dc.subjectcornea inflammationen_US
dc.subjectsyngeneicen_US
dc.subjectallogeneicen_US
dc.titleCorneal Inflammation After Miniature Keratoprosthesis Implantationen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-19T01:30:42Z
dc.description.versionVersion of Recorden_US
dc.relation.journalInvestigative Ophthalmology & Visual Scienceen_US
dash.depositing.authorDana, Reza
dc.date.available2014
dc.date.available2018-02-20T21:44:23Z
dc.identifier.doi10.1167/iovs.14-15884*
workflow.legacycommentscat.completeen_US
dash.authorsorderedfalse
dash.contributor.affiliatedDohlman, Claes
dash.contributor.affiliatedDana, Reza


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