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dc.contributor.authorKeane–Myers, Andrea
dc.contributor.authorMiyazaki, Dai
dc.contributor.authorLiu, Grace
dc.contributor.authorDekaris, Iva
dc.contributor.authorOno, Santa
dc.contributor.authorDana, Reza
dc.date.accessioned2018-02-23T20:21:44Z
dc.date.issued1999
dc.identifierQuick submit: 2017-06-18T18:44:29-0400
dc.identifier.citationAndrea M. Keane–Myers; Dai Miyazaki; Grace Liu; Iva Dekaris; Santa Ono; M. Reza Dana. 1999. Prevention of Allergic Eye Disease by Treatment with IL-1 Receptor Antagonist. Investigative Ophthalmology & Visual Science 40 (12): 3041-6.en_US
dc.identifier.issn0146-0404en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34864854
dc.description.abstractpurpose. To determine the impact of interleukin-1 (IL-1) inhibition using IL-1 receptor antagonist (IL-1Ra) in a mouse model of allergic eye disease. methods. A/J mice sensitized and challenged with cat dander in the eye were treated with topical IL-1Ra or vehicle alone. Control mice were treated with IL-1Ra or vehicle but sensitized and challenged with phosphate-buffered saline alone. Immediately after the final allergen challenge, the mice were observed for behavioral changes and assessed for lid injection and chemosis. The animals were then killed, eyes and attached lids were removed for either RNA extraction or histology, and draining lymph nodes were removed for either RNA extraction or in vitro stimulation assays. Differences in chemokine message between experimental and control groups were determined by RNase protection assays. results. Treatment with IL-1Ra in allergen-challenged animals significantly reduced allergen-induced changes in photosensitivity (60%, P = 0.0002), chemosis (50%, P = 0.0151), and injection (86.7%, P = 0.0068) compared with vehicle-treated controls. Interleukin-1Ra reduced the number of degranulated mast cells and caused a significant reduction in the number of eosinophils infiltrating the conjunctival matrix (P < 0.001) after allergen challenge. Examination of chemokine mRNA taken from the conjunctiva and draining lymph nodes by RNase protection assay showed a profound decrease in the production of a number of C–C chemokines. conclusions. These findings suggest that IL-1Ra is suppressing allergic eye disease by a down-modulation of the recruitment of eosinophils and other inflammatory cells essential for the immunopathogenesis of ocular atopy.en_US
dc.language.isoen_USen_US
dc.publisherAssociation for Research in Vision and Ophthalmologyen_US
dash.licenseLAA
dc.titlePrevention of Allergic Eye Disease by Treatment with IL-1 Receptor Antagonisten_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-18T22:44:31Z
dc.description.versionVersion of Recorden_US
dc.relation.journalInvestigative Ophthalmology & Visual Scienceen_US
dash.depositing.authorDana, Reza
dc.date.available1999
dc.date.available2018-02-23T20:21:44Z
dash.contributor.affiliatedDana, Reza


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