Stereotactically-guided Ablation of the Rat Auditory Cortex, and Localization of the Lesion in the Brain
Merchán, Miguel A.
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CitationLamas, Verónica, Sheila Estévez, Marianni Pernía, Ignacio Plaza, and Miguel A. Merchán. 2017. “Stereotactically-guided Ablation of the Rat Auditory Cortex, and Localization of the Lesion in the Brain.” Journal of Visualized Experiments : JoVE (128): 56429. doi:10.3791/56429. http://dx.doi.org/10.3791/56429.
AbstractThe rat auditory cortex (AC) is becoming popular among auditory neuroscience investigators who are interested in experience-dependence plasticity, auditory perceptual processes, and cortical control of sound processing in the subcortical auditory nuclei. To address new challenges, a procedure to accurately locate and surgically expose the auditory cortex would expedite this research effort. Stereotactic neurosurgery is routinely used in pre-clinical research in animal models to engraft a needle or electrode at a pre-defined location within the auditory cortex. In the following protocol, we use stereotactic methods in a novel way. We identify four coordinate points over the surface of the temporal bone of the rat to define a window that, once opened, accurately exposes both the primary (A1) and secondary (Dorsal and Ventral) cortices of the AC. Using this method, we then perform a surgical ablation of the AC. After such a manipulation is performed, it is necessary to assess the localization, size, and extension of the lesions made in the cortex. Thus, we also describe a method to easily locate the AC ablation postmortem using a coordinate map constructed by transferring the cytoarchitectural limits of the AC to the surface of the brain.The combination of the stereotactically-guided location and ablation of the AC with the localization of the injured area in a coordinate map postmortem facilitates the validation of information obtained from the animal, and leads to a better analysis and comprehension of the data.
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