Conserved epitope on influenza-virus hemagglutinin head defined by a vaccine-induced antibody

DSpace/Manakin Repository

Conserved epitope on influenza-virus hemagglutinin head defined by a vaccine-induced antibody

Citable link to this page

 

 
Title: Conserved epitope on influenza-virus hemagglutinin head defined by a vaccine-induced antibody
Author: Raymond, Donald D.; Bajic, Goran; Ferdman, Jack; Suphaphiphat, Pirada; Settembre, Ethan C.; Moody, M. Anthony; Schmidt, Aaron G.; Harrison, Stephen C.

Note: Order does not necessarily reflect citation order of authors.

Citation: Raymond, Donald D., Goran Bajic, Jack Ferdman, Pirada Suphaphiphat, Ethan C. Settembre, M. Anthony Moody, Aaron G. Schmidt, and Stephen C. Harrison. 2017. “Conserved epitope on influenza-virus hemagglutinin head defined by a vaccine-induced antibody.” Proceedings of the National Academy of Sciences of the United States of America 115 (1): 168-173. doi:10.1073/pnas.1715471115. http://dx.doi.org/10.1073/pnas.1715471115.
Full Text & Related Files:
Abstract: Circulating influenza viruses evade neutralization in their human hosts by acquiring escape mutations at epitopes of prevalent antibodies. A goal for next-generation influenza vaccines is to reduce escape likelihood by selectively eliciting antibodies recognizing conserved surfaces on the viral hemagglutinin (HA). The receptor-binding site (RBS) on the HA “head” and a region near the fusion peptide on the HA “stem” are two such sites. We describe here a human antibody clonal lineage, designated CL6649, members of which bind a third conserved site (“lateral patch”) on the side of the H1-subtype, HA head. A crystal structure of HA with bound Fab6649 shows the conserved antibody footprint. The site was invariant in isolates from 1977 (seasonal) to 2012 (pdm2009); antibodies in CL6649 recognize HAs from the entire period. In 2013, human H1 viruses acquired mutations in this epitope that were retained in subsequent seasons, prompting modification of the H1 vaccine component in 2017. The mutations inhibit Fab6649 binding. We infer from the rapid spread of these mutations in circulating H1 influenza viruses that the previously subdominant, conserved lateral patch had become immunodominant for individuals with B-cell memory imprinted by earlier H1 exposure. We suggest that introduction of the pdm2009 H1 virus, to which most of the broadly prevalent, neutralizing antibodies did not bind, conferred a selective advantage in the immune systems of infected hosts to recall of memory B cells that recognized the lateral patch, the principal exposed epitope that did not change when pdm2009 displaced previous seasonal H1 viruses.
Published Version: doi:10.1073/pnas.1715471115
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5776812/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34868990
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters