Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture
View/ Open
Published Version
https://doi.org/10.1038/s41598-018-19291-4Metadata
Show full item recordCitation
Paik, Elizabeth J., Alison L. O’Neil, Shi-Yan Ng, Chicheng Sun, and Lee L. Rubin. 2018. “Using intracellular markers to identify a novel set of surface markers for live cell purification from a heterogeneous hIPSC culture.” Scientific Reports 8 (1): 804. doi:10.1038/s41598-018-19291-4. http://dx.doi.org/10.1038/s41598-018-19291-4.Abstract
Human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) can provide sources for midbrain dopaminergic (mDA) neural progenitors (NPCs) for cell therapy to treat Parkinson’s disease (PD) patients. However, the well-known line-to-cell line variability in the differentiation capacity of individual cell lines needs to be improved for the success of this therapy. To address this issue, we sought to identify mDA NPC specific cell surface markers for fluorescence activated cell sorting (FACS). Through RNA isolation after sorting for NPCs based on staining for cell-specific transcription factors followed by microarray, we identified two positive cell surface markers (CORIN and CD166) and one negative cell surface marker (CXCR4) for mDA NPC sorting. These three markers can enrich floor plate NPCs to 90% purity, and the sorted NPCs more efficiently differentiate to mature dopaminergic neurons compared to unsorted or CORIN+ alone mDA NPCs. This surface marker identification strategy can be used broadly to facilitate isolation of cell subtypes of interest from heterogeneous cultures.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5770419/pdf/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:34868999
Collections
- FAS Scholarly Articles [18292]
- HMS Scholarly Articles [17922]
Contact administrator regarding this item (to report mistakes or request changes)