X Chromosome Reactivation Dynamics Reveal Stages of Reprogramming to Pluripotency
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Sadhu Dimashkie, Anupama
Plath, KathrinNote: Order does not necessarily reflect citation order of authors.
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CitationPasque, Vincent, Jason Tchieu, Rahul Karnik, Molly Uyeda, Anupama Sadhu Dimashkie, Dana Case, Bernadett Papp, et al. 2014. “X Chromosome Reactivation Dynamics Reveal Stages of Reprogramming to Pluripotency.” Cell 159 (7) (December): 1681–1697. doi:10.1016/j.cell.2014.11.040.
AbstractReprogramming to iPSCs resets the epigenome of somatic cells, including the reversal of X chromosome inactivation. We sought to gain insight into the steps underlying the reprogramming process by examining the means by which reprogramming leads to X chromosome reactivation (XCR). Analyzing single cells in situ, we found that hallmarks of the inactive X (Xi) change sequentially, providing a direct readout of reprogramming progression. Several epigenetic changes on the Xi occur in the inverse order of developmental X inactivation, whereas others are uncoupled from this sequence. Among the latter, DNA methylation has an extraordinary long persistence on the Xi during reprogramming, and, like Xist expression, is erased only after pluripotency genes are activated. Mechanistically, XCR requires both DNA demethylation and Xist silencing, ensuring that only cells undergoing faithful reprogramming initiate XCR. Our study defines the epigenetic state of multiple sequential reprogramming intermediates and establishes a paradigm for studying cell fate transitions during reprogramming.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34878090
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