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dc.contributor.authorPietramaggiori, Giorgio
dc.contributor.authorScherer, Saja S.
dc.contributor.authorCervi, David
dc.contributor.authorKlement, Giannoula
dc.contributor.authorOrgill, Dennis Paul
dc.date.accessioned2018-03-06T21:25:37Z
dc.date.issued2008
dc.identifierQuick submit: 2015-01-05T15:30:04-05:00
dc.identifier.citationPietramaggiori, Giorgio, Saja S. Scherer, David Cervi, Giannoula Klement, and Dennis P. Orgill. 2008. “Tumors Stimulate Platelet Delivery of Angiogenic Factors in Vivo.” The American Journal of Pathology 173 (6) (December): 1609–1616. doi:10.2353/ajpath.2008.080474.en_US
dc.identifier.issn0002-9440en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34900815
dc.description.abstractThe interaction between platelets and the tumor microenvironment results in the modulation of angiogenesis, although the mechanisms governing this regulation remain unclear. This study explores the differences in the communication between wounded tissues and healthy, tumor-conditioned, and frozen platelets. Platelet-rich plasma obtained from healthy (PRP) or tumor-bearing (TPRP) mice was applied to dorsal, full-thickness wounds on diabetic mice. Wound healing was evaluated using macroscopic criteria and a staging system based on angiogenesis and stromal cell proliferation. Proteomic analysis was used to compare the levels of angiogenic proteins contained in the platelet preparations. TPRP-treated wounds reached 90% wound closure 5.6 to 9.5 days earlier than PRP-treated and nontreated wounds, respectively. TPRP induced a fourfold increase in stromal cell proliferation compared with nontreated wounds, and a 2.5-fold increase compared with PRP-treated wounds. TPRP induced the highest stimulation of angiogenesis with a fourfold increase compared with nontreated controls. On day 21, wounds treated with TPRP showed a typical architecture with thick collagen bundles. Although the levels of angiogenesis regulators detected via SELDI-ToF were similar between the PRP and TPRP treatment regimens, the enhanced healing capacity of TPRP suggests improved platelet delivery as indicated by frozen TPRP preparations that had lost most of their pro-angiogenic drive. In conclusion, these results show that intact tumor-conditioned platelets display an improved ability to deliver angiogenesis regulators to wounded tissues.en_US
dc.language.isoen_USen_US
dc.publisherElsevier BVen_US
dc.relation.isversionofdoi:10.2353/ajpath.2008.080474en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pubmed/18988799en_US
dash.licenseLAA
dc.titleTumors Stimulate Platelet Delivery of Angiogenic Factors in Vivoen_US
dc.typeJournal Articleen_US
dc.date.updated2015-01-05T20:30:04Z
dc.description.versionVersion of Recorden_US
dc.rights.holderPietramaggiori G, Scherer SS, Cervi D, Klement G, Orgill DP.
dc.relation.journalThe American Journal of Pathologyen_US
dash.depositing.authorOrgill, Dennis Paul
dc.date.available2018-03-06T21:25:37Z
dc.identifier.doi10.2353/ajpath.2008.080474*
workflow.legacycommentsnoap.needman AAM unavailableen_US
dash.contributor.affiliatedOrgill, Dennis


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