Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery

DSpace/Manakin Repository

Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery

Citable link to this page

 

 
Title: Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery
Author: Gerli, Mattia Francesco Maria; Guyette, Jacques Paul; Evangelista-Leite, Daniele; Ghoshhajra, Brian Burns; Ott, Harald Christian

Note: Order does not necessarily reflect citation order of authors.

Citation: Gerli, Mattia Francesco Maria, Jacques Paul Guyette, Daniele Evangelista-Leite, Brian Burns Ghoshhajra, and Harald Christian Ott. 2018. “Perfusion decellularization of a human limb: A novel platform for composite tissue engineering and reconstructive surgery.” PLoS ONE 13 (1): e0191497. doi:10.1371/journal.pone.0191497. http://dx.doi.org/10.1371/journal.pone.0191497.
Full Text & Related Files:
Abstract: Muscle and fasciocutaneous flaps taken from autologous donor sites are currently the most utilized approach for trauma repair, accounting annually for 4.5 million procedures in the US alone. However, the donor tissue size is limited and the complications related to these surgical techniques lead to morbidities, often involving the donor sites. Alternatively, recent reports indicated that extracellular matrix (ECM) scaffolds boost the regenerative potential of the injured site, as shown in a small cohort of volumetric muscle loss patients. Perfusion decellularization is a bioengineering technology that allows the generation of clinical-scale ECM scaffolds with preserved complex architecture and with an intact vascular template, from a variety of donor organs and tissues. We recently reported that this technology is amenable to generate full composite tissue scaffolds from rat and non-human primate limbs. Translating this platform to human extremities could substantially benefit soft tissue and volumetric muscle loss patients providing tissue- and species-specific grafts. In this proof-of-concept study, we show the successful generation a large-scale, acellular composite tissue scaffold from a full cadaveric human upper extremity. This construct retained its morphological architecture and perfusable vascular conduits. Histological and biochemical validation confirmed the successful removal of nuclear and cellular components, and highlighted the preservation of the native extracellular matrix components. Our results indicate that perfusion decellularization can be applied to produce human composite tissue acellular scaffolds. With its preserved structure and vascular template, these biocompatible constructs, could have significant advantages over the currently implanted matrices by means of nutrient distribution, size-scalability and immunological response.
Published Version: doi:10.1371/journal.pone.0191497
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5774802/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:35014806
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters