Progesterone inhibits contraction and increases TREK-1 potassium channel expression in late pregnant rat uterus

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Progesterone inhibits contraction and increases TREK-1 potassium channel expression in late pregnant rat uterus

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Title: Progesterone inhibits contraction and increases TREK-1 potassium channel expression in late pregnant rat uterus
Author: Yin, Zongzhi; Li, Yun; He, Wenzhu; Li, Dan; Li, Hongyan; Yang, Yuanyuan; Shen, Bing; Wang, Xi; Cao, Yunxia; Khalil, Raouf A.

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Citation: Yin, Zongzhi, Yun Li, Wenzhu He, Dan Li, Hongyan Li, Yuanyuan Yang, Bing Shen, Xi Wang, Yunxia Cao, and Raouf A. Khalil. 2018. “Progesterone inhibits contraction and increases TREK-1 potassium channel expression in late pregnant rat uterus.” Oncotarget 9 (1): 651-661. doi:10.18632/oncotarget.23084. http://dx.doi.org/10.18632/oncotarget.23084.
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Abstract: Objective: The aim of this study was to investigate the effect and mechanism by which progesterone regulates uterine contraction in late pregnant rats Results: Progesterone caused concentration-dependent relaxation of uterine strips that was enhanced compared with control nontreated uterine strips. Uterine strips incubated with progesterone showed a significant increase in TREK-1 mRNA expression and protein level. TREK-1 inhibitor L-methionine partly reversed uterine relaxation caused by the progesterone, while TREK-1 activator arachidonic acid did not cause significant change in progesterone-induced relaxation. Conclusions: Progesterone inhibits uterine contraction and induces uterine relaxation in late pregnancy. The progesterone-induced inhibition of uterine contraction appears to partly involve increased potassium channel TREK-1 expression/activity. Materials and Methods Uterus from late-pregnant rats (gestational day 19) was isolated, and uterine strips were prepared for isometric contraction measurement. Oxytocin-induced contraction was compared in uterine strips pretreated with different concentration of progesterone. TREK-1 potassium channel inhibitor L-methionine and TREK-1 agonist arachidonic acid were used to determine whether the changes caused by progesterone involve changes in TREK-1 activity. The mRNA and protein expression of TREK-1 in uterine tissues were measured using qPCR and Western blot.
Published Version: doi:10.18632/oncotarget.23084
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5787496/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:35014836
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