Cathepsin L expression and regulation in human abdominal aortic aneurysm, atherosclerosis, and vascular cells
View/ Open
Liu_CathepsinLExpressionAndRegulationInHumanAbdominalAorticAneurysmAtherosclerosisAndVascularCells_Atherosclerosis_2006.pdf (685.8Kb)
Access Status
Full text of the requested work is not available in DASH at this time ("dark deposit"). For more information on dark deposits, see our FAQ.Author
Liu, Jian
Yang, Jin-Tian
Ma, Likun
Ren, An
Xu, Wei-Hua
Fu, Huanxiang
Dolganov, Gregory M.
Hu, Chengcheng
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1016/j.atherosclerosis.2005.05.012Metadata
Show full item recordCitation
Liu, Jian, Galina K. Sukhova, Jin-Tian Yang, Jiusong Sun, Likun Ma, An Ren, Wei-Hua Xu, et al. 2006. “Cathepsin L Expression and Regulation in Human Abdominal Aortic Aneurysm, Atherosclerosis, and Vascular Cells.” Atherosclerosis 184 (2) (February): 302–311. doi:10.1016/j.atherosclerosis.2005.05.012.Abstract
The cysteine protease cathepsin L is one of the most potent mammalian elastases and collagenases, widely expressed at basal levels in most tested tissues and cell types, and regulated by pro-inflammatory stimuli. The inflammatory arterial diseases abdominal aortic aneurysm (AAA) and atherosclerosis involve extensive vascular remodeling that requires elastolysis and collagenolysis. This study examined the hypothesis that cathepsin L is over-expressed in human AAA and atherosclerotic lesions and its expression in vascular cell types found in these lesions is regulated by pro-inflammatory cytokines. Immunohistochemical and tissue extract immunoblot analysis demonstrated increased expression of cathepsin L in human AAA and atheromata and localized its expression to lesional smooth muscle cells (SMC), endothelial cells (EC), and macrophages. In primary cultured human SMC, EC, and monocyte-derived macrophages, pro-inflammatory cytokines or growth factors induced the expression of cathepsin L and its activity against extracellular collagen and elastin. Patients with coronary artery stenosis (n = 65) had higher serum cathepsin L levels than those without lesions detectable by quantitative coronary angiography (n = 30) (1.47 ± 0.33 ng/ml versus 0.60 ± 0.06 ng/ml, p < 0.02). A strong correlation between the percent of stenosis of left anterior descending coronary artery and serum cathepsin L levels in patients with stenosis (R = 0.542, p < 0.0001), also suggests involvement of cathepsin L in these vascular diseases.Citable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:35140997
Collections
- HMS Scholarly Articles [17301]
Contact administrator regarding this item (to report mistakes or request changes)
Comments made during the workflow steps
noap.needman