Establishing Chromatin Regulatory Landscape during Mouse Preimplantation Development

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Establishing Chromatin Regulatory Landscape during Mouse Preimplantation Development

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Title: Establishing Chromatin Regulatory Landscape during Mouse Preimplantation Development
Author: Lu, Falong; Liu, Yuting; Inoue, Azusa; Suzuki, Tsukasa; Zhao, Keji; Zhang, Yi

Note: Order does not necessarily reflect citation order of authors.

Citation: Lu, Falong, Yuting Liu, Azusa Inoue, Tsukasa Suzuki, Keji Zhao, and Yi Zhang. 2016. Establishing Chromatin Regulatory Landscape During Mouse Preimplantation Development. Cell 165, no. 6: 1375–1388. doi:10.1016/j.cell.2016.05.050.
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Abstract: How the chromatin regulatory landscape in the inner cell mass cells is established from differentially packaged sperm and egg genomes during preimplantation development is unknown. Here, we develop a low-input DNase I sequencing (liDNase-seq) method that allows us to generate maps of DNase I-hypersensitive site (DHS) of mouse preimplantation embryos from 1-cell to morula stage. The DHS landscape is progressively established with a drastic increase at the 8-cell stage. Paternal chromatin accessibility is quickly reprogrammed after fertilization to the level similar to maternal chromatin, while imprinted genes exhibit allelic accessibility bias. We demonstrate that transcription factor Nfya contributes to zygotic genome activation and DHS formation at the 2-cell stage and that Oct4 contributes to the DHSs gained at the 8-cell stage. Our study reveals the dynamic chromatin regulatory landscape during early development and identifies key transcription factors important for DHS establishment in mammalian embryos.
Published Version: 10.1016/j.cell.2016.05.050
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:35141045
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