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dc.contributor.authorZarlenga, L
dc.contributor.authorGilmore, Michael S.
dc.contributor.authorSahm, D
dc.date.accessioned2018-03-27T20:37:38Z
dc.date.issued1992
dc.identifier.citationZarlenga LJ, Gilmore MS, Sahm DF. 1992. Effects of amino acids on expression of enterococcal vancomycin resistance. Antimicrob Agents Chemother. 36, no. 4: 902-5.en_US
dc.identifier.issn0066-4804en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:35141066
dc.description.abstractThe effects of various amino acids on vancomycin MICs obtained with resistant enterococci was investigated by using broth dilution testing. For both the type A (i.e., possessing transferable resistance to teicoplanin and vancomycin) and the type B (i.e., possessing teicoplanin susceptibility and nontransferable vancomycin resistance) resistant strains, vancomycin MICs in the presence of glycine were substantially lower than those in unsupplemented broth (range of MIC decrease, 8- to 128-fold). No such effect was seen with Enterococcus gallinarum AIB-39 or with the susceptible control Enterococcus faecalis ATCC 29212. Further testing of two type B strains (E. faecalis V583 and V583-2) showed that certain other amino acids (i.e., D-methionine, D-serine, D-alanine, and D-phenylalanine) had effects similar to that of glycine. Results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis with membrane preparations from these strains revealed the production of vancomycin-inducible proteins of sizes comparable to those describe for other enterococcal isolates that exhibit acquired vancomycin resistance. Even in the presence of 0.2 M glycine, the inducible proteins were produced. These results indicate that certain amino acids specifically interfere with the mechanism(s) of acquired vancomycin resistance in enterococci and that the nature of interference probably involves inhibition or circumvention of the inducible proteins' functions.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC189485/pdf/aac00038-0238.pdfen_US
dash.licenseLAA
dc.titleEffects of amino acids on expression of enterococcal vancomycin resistanceen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalAntimicrobial Agents and Chemotherapyen_US
dash.depositing.authorGilmore, Michael S.
dc.date.available2018-03-27T20:37:38Z
workflow.legacycommentsGilmore emailed for aa 5-6-2017en_US
dash.contributor.affiliatedGilmore, Michael


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