Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the Vibrio cholerae O1 Protein Immunome in Adults With Cholera in Bangladesh
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Author
Nakajima, Rie
Liang, Li
Jasinskas, Al
Berger, Amanda
Leung, Daniel T.
Kelly, Meagan
Xu, Peng
Kováč, Pavol
Giffen, Samantha R.
Harbison, James D.
Chowdhury, Fahima
Khan, Ashraful I.
Bhuiyan, Taufiqur Rahman
Felgner, Philip L.
Qadri, Firdausi
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https://doi.org/10.1093/infdis/jix253Metadata
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Charles, R. C., R. Nakajima, L. Liang, A. Jasinskas, A. Berger, D. T. Leung, M. Kelly, et al. 2017. “Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the Vibrio cholerae O1 Protein Immunome in Adults With Cholera in Bangladesh.” The Journal of Infectious Diseases 216 (1): 125-134. doi:10.1093/infdis/jix253. http://dx.doi.org/10.1093/infdis/jix253.Abstract
Abstract Background. Cholera is a severe dehydrating illness of humans caused by toxigenic strains of Vibrio cholerae O1 or O139. Identification of immunogenic V. cholerae antigens could lead to a better understanding of protective immunity in human cholera. Methods. We probed microarrays containing 3652 V. cholerae antigens with plasma and antibody-in-lymphocyte supernatant (ALS, a surrogate marker of mucosal immune responses) from patients with severe cholera caused by V. cholerae O1 in Bangladesh and age-, sex-, and ABO-matched Bangladeshi controls. We validated a subset of identified antigens using enzyme-linked immunosorbent assay. Results. Overall, we identified 608 immunoreactive V. cholerae antigens in our screening, 59 of which had higher immunoreactivity in convalescent compared with acute-stage or healthy control samples (34 in plasma, 39 in mucosal ALS; 13 in both sample sets). Identified antigens included cholera toxin B and A subunits, V. cholerae O–specific polysaccharide and lipopolysaccharide, toxin coregulated pilus A, sialidase, hemolysin A, flagellins (FlaB, FlaC, and FlaD), phosphoenolpyruvate-protein phosphotransferase, and diaminobutyrate–2-oxoglutarate aminotransferase. Conclusions. This study is the first antibody profiling of the mucosal and systemic antibody responses to the nearly complete V. cholerae O1 protein immunome; it has identified antigens that may aid in the development of an improved cholera vaccine.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5853614/pdf/Terms of Use
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