Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV
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Author
Brown, Eric P.
Weiner, Joshua A.
Lin, Shu
Natarajan, Harini
Normandin, Erica
Alter, Galit
Sarzotti-Kelsoe, Marcella
Ackerman, Margaret E.
Published Version
https://doi.org/10.1016/j.jim.2018.01.013Metadata
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Brown, Eric P., Joshua A. Weiner, Shu Lin, Harini Natarajan, Erica Normandin, Dan H. Barouch, Galit Alter, Marcella Sarzotti-Kelsoe, and Margaret E. Ackerman. 2018. “Optimization and qualification of an Fc Array assay for assessments of antibodies against HIV-1/SIV.” Journal of Immunological Methods 455 (1): 24-33. doi:10.1016/j.jim.2018.01.013. http://dx.doi.org/10.1016/j.jim.2018.01.013.Abstract
The Fc Array is a multiplexed assay that assesses the Fc domain characteristics of antigen-specific antibodies with the potential to evaluate up to 500 antigen specificities simultaneously. Antigen-specific antibodies are captured on antigen-conjugated beads and their functional capacity is probed via an array of Fc-binding proteins including antibody subclassing reagents, Fcγ receptors, complement proteins, and lectins. Here we present the results of the optimization and formal qualification of the Fc Array, performed in compliance with Good Clinical Laboratory Practice (GCLP) guidelines. Assay conditions were optimized for performance and reproducibility, and the final version of the assay was then evaluated for specificity, accuracy, precision, limits of detection and quantitation, linearity, range and robustness.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5851662/pdf/Terms of Use
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