dc.contributor.author | Wei, Bryan | |
dc.contributor.author | Dai, Mingjie | |
dc.contributor.author | Yin, Peng | |
dc.date.accessioned | 2018-06-20T16:05:09Z | |
dc.date.issued | 2012 | |
dc.identifier.citation | Wei, Bryan, Mingjie Dai, and Peng Yin. 2012. “Complex Shapes Self-Assembled from Single-Stranded DNA Tiles.” Nature 485 (7400) (May): 623–626. doi:10.1038/nature11075. | en_US |
dc.identifier.issn | 0028-0836 | en_US |
dc.identifier.issn | 1476-4687 | en_US |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:37136781 | |
dc.description.abstract | Programmed self-assembly of strands of nucleic acid has proved highly effective for creating a wide range of structures with desired shapes. A particularly successful implementation is DNA origami, in which a long scaffold strand is folded by hundreds of short auxiliary strands into a complex shape. Modular strategies are in principle simpler and more versatile and have been used to assemble DNA or RNA tiles into periodic and algorithmic5 two-dimensional lattices, extended ribbons and tubes, three-dimensional crystals, polyhedra and simple finite two-dimensional shapes. But creating finite yet complex shapes from a large number of uniquely addressable tiles remains challenging. Here we solve this problem with the simplest tile form, a 'single-stranded tile' (SST) that consists of a 42-base strand of DNA composed entirely of concatenated sticky ends and that binds to four local neighbours during self-assembly. Although ribbons and tubes with controlled circumferences have been created using the SST approach, we extend it to assemble complex two-dimensional shapes and tubes from hundreds (in some cases more than one thousand) distinct tiles. Our main design feature is a self-assembled rectangle that serves as a molecular canvas, with each of its constituent SST strands—folded into a 3 nm-by-7 nm tile and attached to four neighbouring tiles—acting as a pixel. A desired shape, drawn on the canvas, is then produced by one-pot annealing of all those strands that correspond to pixels covered by the target shape; the remaining strands are excluded. We implement the strategy with a master strand collection that corresponds to a 310-pixel canvas, and then use appropriate strand subsets to construct 107 distinct and complex two-dimensional shapes, thereby establishing SST assembly as a simple, modular and robust framework for constructing nanostructures with prescribed shapes from short synthetic DNA strands. | en_US |
dc.description.sponsorship | Other Research Unit | en_US |
dc.language.iso | en_US | en_US |
dc.publisher | Springer Nature | en_US |
dc.relation.isversionof | doi:10.1038/nature11075 | en_US |
dc.relation.hasversion | http://yin.hms.harvard.edu/publications/2012.complexshapes.pdf | en_US |
dash.license | LAA | |
dc.subject | Chemistry | en_US |
dc.title | Complex shapes self-assembled from single-stranded DNA tiles | en_US |
dc.type | Journal Article | en_US |
dc.description.version | Accepted Manuscript | en_US |
dc.relation.journal | Nature | en_US |
dash.depositing.author | Yin, Peng | |
dash.waiver | 2012-01-30 | |
dc.date.available | 2018-06-20T16:05:09Z | |
dash.affiliation.other | Department of Systems Biology, Harvard Medical School | en_US |
dc.identifier.doi | 10.1038/nature11075 | * |
dash.identifier.orcid | 0000-0002-8665-4966 | en_US |
dash.contributor.affiliated | Dai, Mingjie | |
dash.contributor.affiliated | Yin, Peng | |