Mechanism of tandem duplication formation in BRCA1 mutant cells
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Author
Frock, Richard L.
Menghi, Francesca
Duffey, Erin E.
Camacho, Virginia
Hasty, E. Paul
Liu, Edison T.
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https://doi.org/10.1038/nature24477Metadata
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Willis, Nicholas A., Richard L. Frock, Francesca Menghi, Erin E. Duffey, Arvind Panday, Virginia Camacho, E. Paul Hasty, Edison T. Liu, Frederick W. Alt, and Ralph Scully. 2017. “Mechanism of tandem duplication formation in BRCA1 mutant cells.” Nature 551 (7682): 590-595. doi:10.1038/nature24477. http://dx.doi.org/10.1038/nature24477.Abstract
Summary Small ~10 kb microhomology-mediated tandem duplications (“Group 1 TDs”) are abundant in BRCA1-linked but not BRCA2-linked breast cancer genomes. Here, we define the mechanism underlying this “rearrangement signature”. We show that BRCA1, but not BRCA2, suppresses TDs at a Tus/Ter site-specific chromosomal replication fork barrier in primary mammalian cells. BRCA1 has no equivalent role at chromosomal double strand breaks, indicating specificity for the stalled fork response. Tandem duplications in BRCA1 mutant cells arise by a “replication restart-bypass” mechanism terminated by end joining or by microhomology-mediated template switching, the latter forming complex TD breakpoints. We show that solitary DNA ends form directly at Tus/Ter, implicating misrepair of these lesions in TD formation. We find that BRCA1 inactivation is strongly associated with Group 1 TDs in ovarian cancer. The Group 1 TD phenotype may be a general signature of BRCA1-deficient cancer.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5728692/pdf/Terms of Use
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