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dc.contributor.authorKovalenko, Yevgen
dc.contributor.authorSotiri, Irini
dc.contributor.authorTimonen, Jaakko
dc.contributor.authorOverton, Jonathan C.
dc.contributor.authorHolmes, Gareth
dc.contributor.authorAizenberg, Joanna
dc.contributor.authorHowell, Caitlin
dc.date.accessioned2018-07-16T21:01:46Z
dc.date.issued2016
dc.identifierQuick submit: 2018-07-15T11:40:56-0400
dc.identifier.citationKovalenko, Yevgen, Irini Sotiri, Jaakko V. I. Timonen, Jonathan C. Overton, Gareth Holmes, Joanna Aizenberg, and Caitlin Howell. 2016. “Bacterial Interactions with Immobilized Liquid Layers.” Advanced Healthcare Materials 6 (15) (December 8): 1600948. doi:10.1002/adhm.201600948.en_US
dc.identifier.issn2192-2640en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:37235484
dc.description.abstractBacterial interactions with surfaces are at the heart of many infection-related problems in healthcare. In this work, the interactions of clinically-relevant bacteria with immobilized liquid (IL) layers on oil-infused polymers are investigated. Although oil-infused polymers reduce bacterial adhesion in all cases, complex interactions of the bacteria and liquid layer under orbital flow conditions are uncovered. The number of adherent Escherichia coli cells over multiple removal cycles increases in flow compared to static growth conditions, likely due to a disruption of the liquid layer continuity. Surprisingly, however, biofilm formation appears to remain low regardless of growth conditions. No incorporation of the bacteria into the layer is observed. Bacterial type is also found to affect the number of adherent cells, with more E. coli remaining attached under dynamic orbital flow than Staphylococcus aureus, Pseudomonas aeruginosa under identical conditions. Tests with mutant E. coli lacking flagella confirm that flagella play an important role in adhesion to these surfaces. The results presented here shed new light on the interaction of bacteria with IL layers, highlighting the fundamental differences between oil-infused and traditional solid interfaces, as well as providing important information for their eventual translation into materials that reduce bacterial adhesion in medical applications.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.relation.isversionofdoi:10.1002/adhm.201600948en_US
dash.licenseOAP
dc.titleBacterial Interactions with Immobilized Liquid Layersen_US
dc.typeJournal Articleen_US
dc.date.updated2018-07-15T15:40:57Z
dc.description.versionAccepted Manuscripten_US
dc.relation.journalAdvanced Healthcare Materialsen_US
dash.depositing.authorAizenberg, Joanna
dc.date.available2016
dc.date.available2018-07-16T21:01:46Z
dc.identifier.doi10.1002/adhm.201600948*
dash.contributor.affiliatedTimonen, Jaakko
dash.contributor.affiliatedAizenberg, Joanna


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