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dc.contributor.authorDjekidel, Mohamed Nadhiren_US
dc.contributor.authorInoue, Azusaen_US
dc.contributor.authorMatoba, Shogoen_US
dc.contributor.authorSuzuki, Tsukasaen_US
dc.contributor.authorZhang, Chunxiaen_US
dc.contributor.authorLu, Falongen_US
dc.contributor.authorJiang, Lanen_US
dc.contributor.authorZhang, Yien_US
dc.date.accessioned2018-07-25T14:21:38Z
dc.date.issued2018en_US
dc.identifier.citationDjekidel, Mohamed Nadhir, Azusa Inoue, Shogo Matoba, Tsukasa Suzuki, Chunxia Zhang, Falong Lu, Lan Jiang, and Yi Zhang. 2018. “Reprogramming of Chromatin Accessibility in Somatic Cell Nuclear Transfer Is DNA Replication Independent.” Cell reports 23 (7): 1939-1947. doi:10.1016/j.celrep.2018.04.036. http://dx.doi.org/10.1016/j.celrep.2018.04.036.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:37298218
dc.description.abstractSUMMARY Mammalian oocytes have the ability to reset the transcriptional program of differentiated somatic cells into that of totipotent embryos through somatic cell nuclear transfer (SCNT). However, the mechanisms underlying SCNT-mediated reprogramming are largely unknown. To understand the mechanisms governing chromatin reprogramming during SCNT, we profiled DNase I hypersensitive sites (DHSs) in donor cumulus cells and one-cell stage SCNT embryos. To our surprise, the chromatin accessibility landscape of the donor cells is drastically changed to recapitulate that of the in vitro fertilization (IVF)-derived zygotes within 12 hr. Interestingly, this DHS reprogramming takes place even in the presence of a DNA replication inhibitor, suggesting that SCNT-mediated DHS reprogramming is independent of DNA replication. Thus, this study not only reveals the rapid and drastic nature of the changes in chromatin accessibility through SCNT but also establishes a DNA replication-independent model for studying cellular reprogramming.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1016/j.celrep.2018.04.036en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5988247/pdf/en
dash.licenseLAAen_US
dc.titleReprogramming of Chromatin Accessibility in Somatic Cell Nuclear Transfer Is DNA Replication Independenten
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalCell reportsen
dash.depositing.authorZhang, Yien_US
dc.date.available2018-07-25T14:21:38Z
dc.identifier.doi10.1016/j.celrep.2018.04.036*
dash.authorsorderedfalse
dash.contributor.affiliatedZhang, Yi


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