Systematic Analysis of Ribophagy in Human Cells Reveals By-stander Flux During Selective Autophagy
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An, Heeseon, and J. Wade Harper. 2017. “Systematic Analysis of Ribophagy in Human Cells Reveals By-stander Flux During Selective Autophagy.” Nature cell biology 20 (2): 135-143. doi:10.1038/s41556-017-0007-x. http://dx.doi.org/10.1038/s41556-017-0007-x.Abstract
Ribosomes are abundant cellular machines1,2 regulated by assembly, supernumerary subunit turnover, and nascent chain quality control mechanisms1–5. Moreover, nitrogen starvation in yeast has been reported to promote selective ribosome delivery to the vacuole in an autophagy conjugation system-dependent manner, a process called “ribophagy”6,7. However, whether ribophagy in mammals is selective or regulated is unclear. Using Ribo-Keima flux reporters, we find that starvation or mTOR inhibition promotes VPS34-dependent ribophagic flux, which unlike yeast, is largely ATG8 conjugation independent and occurs concomitantly with other cytosolic protein autophagic flux reporters8,9. Ribophagic flux was not induced upon inhibition of translational elongation or nascent chain uncoupling, but was induced in a comparatively selective manner upon proteotoxic stress via arsenite10 or chromosome mis-segregation11 dependent upon VPS34 and ATG8 conjugation. Unexpectedly, agents typically used to induce selective autophagy also promoted increased ribosome and cytosolic protein reporter flux, suggesting significant bulk or “by-stander” autophagy during what is often considered selective autophagy12,13. These results emphasize the importance of monitoring non-specific cargo flux when assessing selective autophagy pathways.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5786475/pdf/Terms of Use
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