Intracellular Protein Scaffolds Enable Simultaneous Measurement of Multiple Biological Signals From Spectrally Identical Fluorescent Sensors
Final version_Johnson_thesis_Jan.docx (6.509Mb)
Access StatusFull text of the requested work is not available in DASH at this time ("dark deposit"). For more information on dark deposits, see our FAQ.
Johnson, Shannon L.
MetadataShow full item record
CitationJohnson, Shannon L. 2020. Intracellular Protein Scaffolds Enable Simultaneous Measurement of Multiple Biological Signals From Spectrally Identical Fluorescent Sensors. Master's thesis, Harvard Extension School.
AbstractBiological signals interact in complex ways within cells, and can exhibit great cell-to-cell heterogeneity as a function of cell history and state. Therefore, there is increasing desire to use multiple fluorescent sensors to simultaneously image multiple biological signals at the same time in individual cells. For decades the limited number of sensors recorded simultaneously has been due to spectral overlap. To circumvent this limitation of spectrally multiplexing sensors, molecular tools for spatially multiplexing have been engineered. Three biological signals were simultaneously measured with spectrally-overlapping sensors using these novel molecular tools. This initial demonstration of the spatial multiplexing strategy opens the door for the simultaneous imaging of dozens of signals within a physiological cascade as more peptide sequences for clustering are designed.
Citable link to this pagehttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37364890
- DCE Theses and Dissertations