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dc.contributor.authorJohnson, Shannon L.
dc.date.accessioned2020-09-11T11:52:45Z
dash.embargo.terms2022-03-01
dc.date.created2020-03
dc.date.issued2020-02-25
dc.date.submitted2020
dc.identifier.citationJohnson, Shannon L. 2020. Intracellular Protein Scaffolds Enable Simultaneous Measurement of Multiple Biological Signals From Spectrally Identical Fluorescent Sensors. Master's thesis, Harvard Extension School.
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37364890*
dc.description.abstractBiological signals interact in complex ways within cells, and can exhibit great cell-to-cell heterogeneity as a function of cell history and state. Therefore, there is increasing desire to use multiple fluorescent sensors to simultaneously image multiple biological signals at the same time in individual cells. For decades the limited number of sensors recorded simultaneously has been due to spectral overlap. To circumvent this limitation of spectrally multiplexing sensors, molecular tools for spatially multiplexing have been engineered. Three biological signals were simultaneously measured with spectrally-overlapping sensors using these novel molecular tools. This initial demonstration of the spatial multiplexing strategy opens the door for the simultaneous imaging of dozens of signals within a physiological cascade as more peptide sequences for clustering are designed.
dc.description.sponsorshipBioengineering & Nanotechnology
dc.format.mimetypeapplication/pdf
dash.licenseLAA
dc.subjectspatially multiplexed imaging
dc.subjectgenetically encoded sensors
dc.subjectfluorescent sensors
dc.subjectlive cell imaging
dc.subjectprotein scaffold
dc.subjectcalcium imaging
dc.subjectcAMP
dc.subjectPKA
dc.subjectmammalian cells
dc.subjectneuron
dc.titleIntracellular Protein Scaffolds Enable Simultaneous Measurement of Multiple Biological Signals From Spectrally Identical Fluorescent Sensors
dc.typeThesis or Dissertation
dash.depositing.authorJohnson, Shannon L.
dash.embargo.until2022-03-01
dc.date.available2020-09-11T11:52:45Z
thesis.degree.date2020
thesis.degree.grantorHarvard Extension School
thesis.degree.grantorHarvard Extension School
thesis.degree.levelMasters
thesis.degree.levelMasters
thesis.degree.nameALM
thesis.degree.nameALM
dc.contributor.committeeMemberDenkin, Steven
dc.contributor.committeeMemberBoyden, Ed
dc.type.materialtext
thesis.degree.departmentBioengineering & Nanotechnology
thesis.degree.departmentBioengineering & Nanotechnology
dash.identifier.vireo
dc.identifier.orcid0000-0002-6758-224X
dash.author.emailshannon_johnson@alumni.brown.edu


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