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dc.contributor.authorQatarneh, Alia Yahya
dc.date.accessioned2020-09-14T15:28:32Z
dc.date.created2020-05
dc.date.issued2020-04-14
dc.date.submitted2020
dc.identifier.citationQatarneh, Alia Yahya. 2020. Establishing the Freeze-Dried Cell-Free Protein Synthesis System as a Paradigm for Studying Protein Folding on Earth and in Microgravity. Master's thesis, Harvard Extension School.
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37365071*
dc.description.abstractAs virtually all fundamental processes for biological life are executed by proteins, the study of protein folding and folding kinetics is essential. In order to advance the study of protein folding in resource-limited settings, it is necessary to develop easy to use, inexpensive, and accessible assays to study protein folding, unfolding, stability, and kinetics. Through this research, I sought to develop a low-resource module that explores the parameters of protein folding in an open, cell-free system. The work proceeded in three stages: to establish an experimental set up, adapt said experimental set up for use in educational settings, and finally to lay the groundwork for space application. Here we show that the study of folding kinetics and the structural stability of fluorescent proteins can be achieved through the freeze-dried, cell-free platform called BioBitsⓇ. Combined, this research brings an enhanced study of protein synthesis and folding to the bench, the classroom, and to space.
dc.description.sponsorshipBiotechnology
dc.format.mimetypeapplication/pdf
dash.licenseLAA
dc.subjectBioBits®, Biotechnology, Central Dogma, Cell-Free Protein Synthesis (CFPS), Education, Freeze-Dried Cell-Free (FD-CF), Fluorescent proteins, Folding kinetics, GFP, GFPmut3b, Microgravity, miniPCR™, P51™, Protein denaturation, Protein folding, Protein synthesis, sfGFP, Synthetic biology
dc.titleEstablishing the Freeze-Dried Cell-Free Protein Synthesis System as a Paradigm for Studying Protein Folding on Earth and in Microgravity
dc.typeThesis or Dissertation
dash.depositing.authorQatarneh, Alia Yahya
dc.date.available2020-09-14T15:28:32Z
thesis.degree.date2020
thesis.degree.grantorHarvard Extension School
thesis.degree.grantorHarvard Extension School
thesis.degree.levelMasters
thesis.degree.levelMasters
thesis.degree.nameALM
thesis.degree.nameALM
dc.contributor.committeeMemberKraves, Sebastian
dc.type.materialtext
thesis.degree.departmentBiotechnology
thesis.degree.departmentBiotechnology
dash.identifier.vireo
dc.identifier.orcid0000-0002-6999-9831
dash.author.emailaliaqatarneh@fas.harvard.edu


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