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dc.contributor.advisorNeedleman, Daniel
dc.contributor.advisorBasan, Markus
dc.contributor.advisorLaub, Michael
dc.contributor.authorBarber, Felix
dc.date.accessioned2020-09-15T10:45:30Z
dc.date.created2020-03
dc.date.issued2020-01-22
dc.date.submitted2020
dc.identifier.citationBarber, Felix. 2020. The interplay between cell size control, cell cycle regulation, and growth morphology in budding yeast. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences.
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37365167*
dc.description.abstractOrganisms from all domains of life display size control, coupling growth and division to constrain the spread in cell sizes observed throughout a population. However, the means by which this coupling takes place remains unclear. Here we present our work studying the molecular mechanism underpinning size control using a combination of theoretical and experimental approaches. First we explore a set of abstracted molecular models and their implications for the regulation of size control in different growth morphologies. This approach revealed rich dependence of the robustness of different strategies on division asymmetry and growth morphology. We then use a series of microscopy-based experiments to interrogate the molecular mechanism underpinning cell size control in the model organism budding yeast S. cerevisiae. Within this work we disprove a widely supported hypothesis for size control in this organism, highlighting the challenges that remain in addressing the fundamental question of how cells regulate their size. Finally, we use a theoretical approach to address the question of how cell size and division asymmetry affect the population growth rate. This project demonstrates a previously unobserved dependence of population growth rate on division asymmetry and a cell's strategy of size control.
dc.description.sponsorshipBiology, Molecular and Cellular
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectWhi5
dc.subjectStart
dc.subjectcell size control
dc.subjectbudding yeast
dc.subjectsingle-cell time-lapse microscopy
dc.subjectmodeling
dc.subjectstochastic processes
dc.titleThe interplay between cell size control, cell cycle regulation, and growth morphology in budding yeast
dc.typeThesis or Dissertation
dash.depositing.authorBarber, Felix
dc.date.available2020-09-15T10:45:30Z
thesis.degree.date2020
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.levelDoctoral
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
thesis.degree.nameDoctor of Philosophy
dc.type.materialtext
thesis.degree.departmentBiology, Molecular and Cellular
thesis.degree.departmentBiology, Molecular and Cellular
dash.identifier.vireo
dc.identifier.orcid0000-0003-1252-5181
dash.author.emailfelix1635@gmail.com


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