The Role of Interferon α in human T peripheral helper cells and T follicular helper cells
Pena Nunez, Diana
MetadataShow full item record
CitationPena Nunez, Diana. 2020. The Role of Interferon α in human T peripheral helper cells and T follicular helper cells. Master's thesis, Harvard Medical School.
AbstractA strong correlation has been found between the interferon signature and the pathogenic expansion of Tph and Tfh cells in lupus. Still, the roles of the Type I IFN on the expression of transcription factors such as MAF and BCL6, and its role on Tph cells in humans is unclear.
Through multiple in vitro approaches, CD4 T cells were activated in the presence of IFNα and other cytokines. We analyze through flow cytometry the expression of PD1, CXCR5, ICOS, and HLA-DR as well as some transcription factors present in Tfh and Tph cells. We also evaluated the production of CXCL13 and IL-21 by ELISA. We found that IFNα affected the expression of some surface markers and transcription factors in human T helper cells; moreover, IFNα up-regulated CXCL13 expression in CD4 T cells.
This data provide further support to the idea that type I IFN might be one of the drivers of the expansion of the pathogenic Tfh or Tph cells or the aberrant T and B cell interaction in lupus. This effect may be one of the mechanisms through which emerging therapies that block IFN, such as anifrolumab, may blunt the pathologic immune response in lupus.
Citable link to this pagehttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37365276