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dc.contributor.advisorNeedleman, Daniel J.
dc.contributor.authorWu, Hai-Yin
dc.date.accessioned2020-10-16T14:36:48Z
dc.date.created2020-05
dc.date.issued2020-05-15
dc.date.submitted2020
dc.identifier.citationWu, Hai-Yin. 2020. Biophysics of Mitotic Spindle Positioning in Caenorhabditis elegans Early Embryos. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences.
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37365918*
dc.description.abstractIn the C. elegans early embryo, the pronuclei complex migrates to the cell center and rotates such that the mitotic spindle forms along the anterior-posterior axis. Subsequently, the spindle undergoes transverse oscillations as it moves toward the posterior. This asymmetric spindle positioning is crucial for asymmetric cell division and proper development. The contributions of pushing, cortical pulling, and cytoplasmic pulling forces to those dynamic centrosome positioning events are not fully understood. To study these processes, we constructed a novel laser ablation system capable of creating nearly arbitrary 3D cuts of astral microtubules at any desirable timing. We used this system to dissect the relative contribution of pushing and pulling forces throughout pronuclei and spindle motions. Our results suggest that all of these motions are dominated by net pulling. We used microinjected fluorescent nanodiamonds to track cytoplasmic fluid flow, which indicates that cortical pulling forces dominate over cytoplasmic pulling at all stages. We used mathematical modeling and computer simulations to interpret our experimental data. Taken together, our results strongly argue that cortical pulling drives pronuclear migration and rotation, metaphase spindle positioning, asymmetric spindle positioning and all aspects of spindle oscillations.
dc.description.sponsorshipPhysics
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectcell division
dc.subjectmitosis
dc.subjectmicrotubule(MT)
dc.subjectastral microtubule
dc.subjectcentrosome
dc.subjectspindle positioning
dc.subjectlaser ablation
dc.subjectfluorescent nanodiamond(FND)
dc.subjectpronuclear migration and rotation
dc.subjectspindle oscillation
dc.subjectpushing force
dc.subjectcortical pulling force
dc.subjectcytoplasmic pulling force
dc.titleBiophysics of Mitotic Spindle Positioning in Caenorhabditis elegans Early Embryos
dc.typeThesis or Dissertation
dash.depositing.authorWu, Hai-Yin
dc.date.available2020-10-16T14:36:48Z
thesis.degree.date2020
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.levelDoctoral
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
thesis.degree.nameDoctor of Philosophy
dc.contributor.committeeMemberManoharan, Vinothan N.
dc.contributor.committeeMemberSamuel, Aravinthan
dc.type.materialtext
thesis.degree.departmentPhysics
thesis.degree.departmentPhysics
dash.identifier.vireo
dc.identifier.orcid0000-0001-8123-069X
dash.author.emailhaiyinwu@gmail.com


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