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dc.contributor.authorDion, Michael
dc.contributor.authorKapoor, Mrinal
dc.contributor.authorSun, Yingjie
dc.contributor.authorWilson, Sean
dc.contributor.authorRyan, Joel
dc.contributor.authorVigouroux, Antoine
dc.contributor.authorvan Teeffelen, Sven
dc.contributor.authorOldenbourg, Rudolf
dc.contributor.authorGarner, Ethan
dc.date.accessioned2020-12-10T16:00:17Z
dc.date.issued2019-05-13
dc.identifier.citationDion, M.F., Kapoor, M., Sun, Y. et al. Bacillus subtilis cell diameter is determined by the opposing actions of two distinct cell wall synthetic systems. Nat Microbiol 4, 1294–1305 (2019). https://doi.org/10.1038/s41564-019-0439-0en_US
dc.identifier.issn2058-5276en_US
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37366567*
dc.description.abstractRod shaped bacteria grow by adding material into their cell wall via the action of two spatially distinct enzymatic systems: The Rod system moves around the cell circumference, while the class A penicillin-binding proteins (aPBPs) are unorganized. To understand how the combined action of these two systems defines bacterial dimensions, we examined how each system affects the growth and width of Bacillus subtilis, as well as the mechanical anisotropy and orientation of material within their sacculi. We find that rod diameter is not determined by MreB, rather it depends on the balance between the systems: The Rod system reduces diameter, while aPBPs increase it. RodA/PBP2A can both thin or widen cells, depending on its levels relative to MreBCD. Increased Rod system activity correlates with an increased density of directional MreB filaments, and a greater fraction of directionally moving PBP2A molecules. This increased circumferential synthesis increases the amount of oriented material within the sacculi, increasing their mechanical anisotropy and reinforcing rod shape. Together, these experiments explain how the combined action of the two main cell wall synthetic systems build rods of different widths, a model that appears generalizable: Escherichia coli containing Rod system mutants show the same relationship between the density of directionally moving MreB filaments and cell width.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relationNature Microbiologyen_US
dash.licenseLAA
dc.subjectImmunologyen_US
dc.subjectMicrobiology (medical)en_US
dc.subjectApplied Microbiology and Biotechnologyen_US
dc.subjectGeneticsen_US
dc.subjectCell Biologyen_US
dc.subjectMicrobiologyen_US
dc.titleBacillus subtilis cell diameter is determined by the opposing actions of two distinct cell wall synthetic systemsen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Microbiologyen_US
dash.depositing.authorGarner, Ethan
dash.waiver2019-03-26
dc.date.available2020-12-10T16:00:17Z
dash.affiliation.otherHarvard Medical Schoolen_US
dc.identifier.doi10.1038/s41564-019-0439-0
dc.source.journalNat Microbiol
dash.waiver.reasonRequested by publisheren_US
dash.source.volume4;8
dash.source.page1294-1305
dash.contributor.affiliatedWilson, Sean
dash.contributor.affiliatedSun, Yingjie
dash.contributor.affiliatedGarner, Ethan


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