The Twin Spot Generator for Differential Drosophila Lineage Analysis
del Valle Rodriguez, Alberto
Hohl, Amber M
Bateman, Jack R
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CitationGriffin, Ruth, Anne Sustar, Marianne Bonvin, Richard Binari, Alberto del Valle Rodriguez, Amber M. Hohl, Jack R. Bateman et al. 2009. "The twin spot generator for differential Drosophila lineage analysis." Nature Methods 6, no. 8: 600-602.
AbstractIn Drosophila, widely-used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the Twin Spot Generator technique (TSG) and demonstrate that through mitotic recombination, TSG generates green and red twin spots in internal fly tissues, visible even as single cells. We discuss the wide applications of TSG to lineage and genetic mosaic studies.
Citable link to this pagehttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37367089
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