Rasp, a Putative Transmembrane Acyltransferase, Is Required for Hedgehog Signaling
View/ Open
rasp, a putative transmembrane acyltransferase, is required for Hedgehog signaling.pdf (419.2Kb)
Access Status
Full text of the requested work is not available in DASH at this time ("restricted access"). For more information on restricted deposits, see our FAQ.Metadata
Show full item recordCitation
Micchelli, Craig A., Erica Selva, Vladic Mogila, and Norbert Perrimon. "Rasp, a putative transmembrane acyltransferase, is required for Hedgehog signaling." Development 129, no. 4 (2002): 843-851.Abstract
Members of the Hedgehog (Hh) family encode secreted molecules that act as potent organizers during vertebrate and invertebrate development. Post-translational modification regulates both the range and efficacy of Hh protein. One such modification is the acylation of the N- terminal cysteine of Hh. In a screen for zygotic lethal mutations associated with maternal effects, we have identified rasp, a novel Drosophila segment polarity gene. Analysis of the rasp mutant phenotype, in both the embryo and wing imaginal disc demonstrates that rasp does not disrupt Wnt/Wingless signaling but is specifically required for Hh signaling. The requirement of rasp is restricted only to those cells that produce Hh; hh transcription, protein levels and distribution are not affected by the loss of rasp. Molecular analysis reveals that rasp encodes a multipass transmembrane protein that has homology to a family of membrane bound O-acyl transferases. Our results suggest that Rasp-dependent acylation is necessary to generate a fully active Hh protein.Citable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37367166
Collections
- HMS Scholarly Articles [17922]
Contact administrator regarding this item (to report mistakes or request changes)