Xio is a Component of the Drosophila Sex Determination Pathway and RNA N6-Methyladenosine Methyltransferase Complex
View/ Open
Xio is a component of the Drosophila sex determination pathway and RNA N6-methyladenosine methyltransferase complex.pdf (1.475Mb)
Access Status
Full text of the requested work is not available in DASH at this time ("restricted access"). For more information on restricted deposits, see our FAQ.Published Version
https://doi.org/10.1073/pnas.1720945115Metadata
Show full item recordCitation
Guo, Jian, Hong-Wen Tang, Jing Li, Norbert Perrimon, Dong Yan. "Xio is a Component of the Drosophila Sex Determination Pathway and RNA N6-Methyladenosine Methyltransferase Complex." Proceedings of the National Academy of Sciences 115, no. 14 (2018): 3674-3679. DOI: 10.1073/pnas.1720945115Abstract
N6-methyladenosine (m6A), the most abundant chemical modification in eukaryotic mRNA, has been implicated in Drosophila sex determination by modifying Sex-lethal (Sxl) pre-mRNA and facilitating its alternative splicing. Here, we identify a sex determination gene, CG7358, and rename it xio according to its loss-of-function female-to-male transformation phenotype. xio encodes a conserved ubiquitous nuclear protein of unknown function. We show that Xio colocalizes and interacts with all previously known m6A writer complex subunits (METTL3, METTL14, Fl(2)d/WTAP, Vir/KIAA1429, and Nito/Rbm15) and that loss of xio is associated with phenotypes that resemble other m6A factors, such as sexual transformations, Sxl splicing defect, held-out wings, flightless flies, and reduction of m6A levels. Thus, Xio encodes a member of the m6A methyltransferase complex involved in mRNA modification. Since its ortholog ZC3H13 (or KIAA0853) also associates with several m6A writer factors, the function of Xio in the m6A pathway is likely evolutionarily conserved.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5889661/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369612
Collections
- HMS Scholarly Articles [17875]
Contact administrator regarding this item (to report mistakes or request changes)