Optimized Gene Editing Technology for Drosophila melanogaster Using Germ Line-Specific Cas9
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CitationRen, Xingjie, Jin Sun, Benjamin Housden, Yanhui Hu, Charles Roesel, Shuailiang Lin, Lu-Ping Liu et al. "Optimized Gene Editing Technology for Drosophila melanogaster Using Germ Line-Specific Cas9." Proceedings of the National Academy of Sciences 110, no. 47 (2013): 19012-19017. DOI: 10.1073/pnas.1318481110
AbstractThe ability to engineer genomes in a specific, systematic, and cost- effective way is critical for functional genomic studies. Recent advances using the CRISPR-associated single-guide RNA system (Cas9/sgRNA) illustrate the potential of this simple system for ge- nome engineering in a number of organisms. Here we report an effective and inexpensive method for genome DNA editing in Drosophila melanogaster whereby plasmid DNAs encoding short sgRNAs under the control of the U6b promoter are injected into transgenic flies in which Cas9 is specifically expressed in the germ line via the nanos promoter. We evaluate the off-targets associ- ated with the method and establish a Web-based resource, along with a searchable, genome-wide database of predicted sgRNAs appropriate for genome engineering in flies. Finally, we discuss the advantages of our method in comparison with other recently published approaches.
Citable link to this pagehttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369613
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