Genome-Wide RNAi Screen Reveals a Specific Sensitivity of IRES-Containing RNA Viruses to Host Translation Inhibition
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CitationCherry, Sara, Tammy Doukas, Susan Armknecht, Sean Whelan, Hui Wang, Peter Sarnow, Norbert Perrimon. "Genome-Wide RNAi Screen Reveals a Specific Sensitivity of IRES-Containing RNA Viruses to Host Translation Inhibition." Genes and Development 19, no. 4 (2005): 445-452. DOI: 10.1101/gad.1267905
AbstractThe widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.
Citable link to this pagehttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369715
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