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dc.contributor.authorHe, Li
dc.contributor.authorHuang, Jiuhong
dc.contributor.authorPerrimon, Norbert
dc.date.accessioned2021-10-08T12:55:33Z
dc.date.issued2017-05-23
dc.identifier.citationHe, Li, Jiuhong Huang, Norbert Perrimon. "Development of an Optimized Synthetic Notch Receptor as an in Vivo Cell–Cell Contact Sensor." Proceedings of the National Academy of Sciences 114, no. 21 (2017): 5467-5472. DOI: 10.1073/pnas.1703205114
dc.identifier.issn0027-8424en_US
dc.identifier.issn1091-6490en_US
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369726*
dc.description.abstractDetection and manipulation of direct cell–cell contact in complex tissues is a fundamental and challenging problem in many biological studies. Here, we report an optimized Notch-based synthetic receptor (synNQ) useful to study direct cell–cell interactions in Drosophila. With the synNQ system, cells expressing a synthetic receptor, which contains Notch activation machinery and a downstream transcriptional activator, QF, are activated by a synthetic GFP ligand expressed by contacting neighbor cells. To avoid cis-inhibition, mutually exclusive expression of the synthetic ligand and receptor is achieved using the “flippase-out” system. Expression of the synthetic GFP ligand is controlled by the Gal4/UAS system for easy and broad applications. Using synNQ, we successfully visualized cell–cell interactions within and between most fly tissues, revealing previously undocumented cell–cell contacts. Importantly, in addition to detection of cells in contact with one another, synNQ allows for genetic manipulation in all cells in contact with a targeted cell population, which we demonstrate in the context of cell competition in developing wing disks. Altogether, the synNQ genetic system will enable a broad range of studies of cell contact in developmental biology.en_US
dc.language.isoen_USen_US
dc.publisherNational Academy of Sciencesen_US
dc.relation.isversionofdoi:10.1073/pnas.1703205114en_US
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5448223/en_US
dash.licenseMETA_ONLY
dc.subjectResearch Subject Categories::NATURAL SCIENCES::Biology::Organism biology::Developmental biologyen_US
dc.titleDevelopment of an Optimized Synthetic Notch Receptor as an in Vivo Cell–Cell Contact Sensoren_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalProceedings of the National Academy of Sciencesen_US
dash.depositing.authorPerrimon, Norbert
dc.date.available2021-10-08T12:55:33Z
dc.identifier.doi10.1073/pnas.1703205114
dc.source.journalProc Natl Acad Sci USA
dash.source.volume114en_US
dash.source.page5467-5472en_US
dash.source.issue21en_US
dash.contributor.affiliatedPerrimon, Norbert


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