Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses
Author
Neumuller, Ralph
Gross, Thomas
Samsonova, Anastasia A.
Vinayagam, Arunachalam
Buckner, Michael
Founk, Karen
Sharifpoor, Sara
Rosebrock, Adam P.
Andrews, Brenda
Published Version
https://doi.org/10.1126/scisignal.2004145Metadata
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Neumuller, Ralph, Thomas Gross, Anastasia A. Samsonova, Arunachalam Vinayagam, Michael Buckner, Karen Founk, Yanhui Hu et al. "Conserved Regulators of Nucleolar Size Revealed by Global Phenotypic Analyses." Science Signaling 6, no. 289 (2013): ra70. DOI: 10.1126/scisignal.2004145Abstract
Regulation of cell growth is a fundamental process in development and disease that integrates a vast array of extra- and intracellular information. A central player in this process is RNA polymerase I (Pol I), which transcribes ribosomal RNA (rRNA) genes in the nucleolus. Rapidly growing cancer cells are characterized by increased Pol I–mediated transcription and, consequently, nucleolar hypertrophy. To map the genetic network underlying the regulation of nucleolar size and of Pol I–mediated transcription, we performed comparative, genome-wide loss-of-function analyses of nucleolar size in Saccharomyces cerevisiae and Drosophila melanogaster coupled with mass spectrometry–based analyses of the ribosomal DNA (rDNA) promoter. With this approach, we identified a set of conserved and nonconserved molecular complexes that control nucleolar size. Furthermore, we characterized a direct role of the histone information regulator (HIR) complex in repressing rRNA transcription in yeast. Our study provides a full-genome, cross-species analysis of a nuclear subcompartment and shows that this approach can identify conserved molecular modules.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3964804/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37369733
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