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dc.contributor.authorMarkstein, Michele
dc.contributor.authorPitsouli, Chrysoula
dc.contributor.authorVillalta, Christians
dc.contributor.authorCelniker, Susan E.
dc.contributor.authorPerrimon, Norbert
dc.date.accessioned2022-06-27T16:03:13Z
dc.date.issued2008-03-02
dc.identifier.citationMarkstein, Michele, Chrysoula Pitsouli, Christians Villalta, Susan E. Celniker, Norbert Perrimon. "Exploiting Position Effects and the Gypsy Retrovirus Insulator to Engineer Precisely Expressed Transgenes." Nature Genetics 40, no. 4 (2008): 476-483. DOI: 10.1038/ng.101
dc.identifier.issn1061-4036en_US
dc.identifier.issn1546-1718en_US
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372411*
dc.description.abstractA major obstacle to creating precisely expressed transgenes lies in the epigenetic effects of the host chromatin that surrounds them. Here we present a strategy to overcome this problem, employing a Gal4-inducible luciferase assay to systematically quantify position effects of host chromatin and the ability of insulators to counteract these effects at phiC31 integration loci randomly distributed throughout the Drosophila genome. We identify loci that can be exploited to deliver precise doses of transgene expression to specific tissues. Moreover, we uncover a previously unrecognized property of the gypsy retrovirus insulator to boost gene expression to levels severalfold greater than at most or possibly all un-insulated loci, in every tissue tested. These findings provide the first opportunity to create a battery of transgenes that can be reliably expressed at high levels in virtually any tissue by integration at a single locus, and conversely, to engineer a controlled phenotypic allelic series by exploiting several loci. The generality of our approach makes it adaptable to other model systems to identify and modify loci for optimal transgene expression.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relation.isversionofdoi:10.1038/ng.101en_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2330261/en_US
dash.licenseLAA
dc.subjectGeneticsen_US
dc.titleExploiting Position Effects and the Gypsy Retrovirus Insulator to Engineer Precisely Expressed Transgenesen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Geneticsen_US
dash.depositing.authorPerrimon, Norbert
dc.date.available2022-06-27T16:03:13Z
dc.identifier.doi10.1038/ng.101
dc.source.journalNat Genet
dash.source.volume40en_US
dash.source.page476-483en_US
dash.source.issue4en_US
dash.contributor.affiliatedVillalta, Christians
dash.contributor.affiliatedPerrimon, Norbert


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