B Cells Populating the Multiple Sclerosis Brain Mature in the Draining Cervical Lymph Nodes
Author
Stern, Joel N. H.
Yaari, Gur
Vander Heiden, Jason A.
Donahue, William F.
Hintzen, Rogier Q.
Huttner, Anita J.
Laman, Jon D.
Nagra, Rashed M.
Nylander, Alyssa
Pitt, David
Ramanan, Sriram
Siddiqui, Bilal A.
Vigneault, Francois
Kleinstein, Steven H.
Hafler, David A.
O’Connor, Kevin C.
Published Version
https://doi.org/10.1126/scitranslmed.3008879Metadata
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Stern, Joel N. H., Gur Yaari, Jason A. Vander Heiden, George Church, William F. Donahue, Rogier Q. Hintzen, Anita J. Huttner et al. "B Cells Populating the Multiple Sclerosis Brain Mature in the Draining Cervical Lymph Nodes." Science Translational Medicine 6, no. 248 (2014): 248ra107. DOI: 10.1126/scitranslmed.3008879Abstract
Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS) characterized by autoimmune mediated demyelination and neurodegeneration. The CNS of patients with MS harbors expanded clones of antigen-experienced B cells that reside in distinct compartments including the meninges, cerebrospinal fluid (CSF) and parenchyma. It is not understood whether this immune infiltrate initiates its development in the CNS or in peripheral tissues. B cells in the CSF can exchange with those in peripheral blood, implying that CNS B cells may have access to lymphoid tissue that may be the specific compartment(s) in which CNS resident B cells encounter antigen and experience affinity maturation. In this study, paired tissues were used to determine whether the B cells that populate the CNS mature in the draining cervical lymph nodes (CLNs). High-throughput sequencing of the antibody repertoire demonstrated that clonally expanded B cells were present in both compartments. Founding members of clonal families were more often found in the draining CLNs. More mature clonal family members derived from these founders were observed in the draining CLNs and also in the CNS, including lesions. These data provide new evidence that B cells traffic freely across the tissue barrier with the majority of B cell maturation occurring outside of the CNS in the secondary lymphoid tissue. Our study may aid in further defining the mechanisms of immunomodulatory therapies that either deplete circulating B cells or impact the intrathecal B cell compartment by inhibiting lymphocyte transmigration into the CNS.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4388137/Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37372860
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