Show simple item record

dc.contributor.authorDi Stefano, Bruno
dc.contributor.authorStik, Grégoire
dc.contributor.authorVila-Casadesús, Maria
dc.contributor.authorSardina, José Luis
dc.contributor.authorVidal, Enrique
dc.contributor.authorDasti, Alessandro
dc.contributor.authorSegura Morales, Carolina
dc.contributor.authorDe Andres Aguayo, Luisa
dc.contributor.authorGomez, Antonio
dc.contributor.authorGoldmann, Johanna
dc.contributor.authorJaenisch, Rudolf
dc.contributor.authorGraf, Thomas
dc.contributor.authorTian, Tian
dc.contributor.authorJaenisch, Rudolf
dc.date.accessioned2023-02-07T13:43:05Z
dc.date.issued2019-06-24
dc.identifier.citationTian, Tian V., Bruno Di Stefano, Grégoire Stik, Maria Vila-Casadesús, José Luis Sardina, Enrique Vidal, Alessandro Dasti, Carolina Segura-Morales, Luisa De Andrés-Aguayo, Antonio Gómez, Johanna Goldmann, Rudolf Jaenisch, and Thomas Graf. 2019. Whsc1 Links Pluripotency Exit with Mesendoderm Specification. Nature Cell Biology 21, no. 7: 824-34.en_US
dc.identifier.issn1476-4679en_US
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37374212*
dc.description.abstractHow pluripotent stem cells differentiate into the main germ layers is a key question of developmental biology. Here we show that the chromatin-related factor Whsc1 (Nsd2, MMSET) has a dual role in pluripotency exit and germ layer specification of embryonic stem cells (ESCs). Upon induction of differentiation, a proportion of Whsc1-depleted ESCs remain entrapped in a pluripotent state and fail to form mesendoderm, although they are still capable of generating neuroectoderm. These functions of Whsc1 are independent of its methyltransferase activity. Whsc1 binds to enhancers of the mesendodermal regulators Gata4, T (Brachyury), Gata6 and Foxa2 together with Brd4, and activates the genes’ expression. Depleting each of these regulators also delays pluripotency exit, suggesting that they mediate the effects observed with Whsc1. Our data indicate that Whsc1 links silencing of the pluripotency regulatory network with activation of mesendoderm lineages.en_US
dc.language.isoen_USen_US
dc.publisherNature Publishing Groupen_US
dc.relationNature Cell Biologyen_US
dash.licenseMETA_ONLY
dc.titleWhsc1 Links Pluripotency Exit With Mesendoderm Specificationen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Cell Biologyen_US
dash.waiver2019-04-19
dc.date.available2023-02-07T13:43:05Z
dash.affiliation.otherHarvard Medical Schoolen_US
dash.waiver.reasonThe Harvard Open Access publishing policy is incompatible with Nature Cell Biology policy.en_US
dash.contributor.affiliatedDi Stefano, Bruno


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record