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dc.contributor.authorTang, Tzu-Chieh
dc.contributor.authorTham, Eléonore
dc.contributor.authorLiu, Xinyue
dc.contributor.authorYehl, Kevin
dc.contributor.authorRovner, Alexis
dc.contributor.authorYuk, Hyunwoo
dc.contributor.authorde la Fuente-Nunez, Cesar
dc.contributor.authorIsaacs, Farren
dc.contributor.authorZhao, Xuanhe
dc.contributor.authorLu, Timothy K.
dc.contributor.authorLu, Timothy
dc.date.accessioned2023-02-10T14:15:43Z
dc.date.issued2021-04-05
dc.identifier.citationTang, Tzu-Chieh, Eléonore Tham, Xinyue Liu, Kevin Yehl, Alexis J Rovner, Hyunwoo Yuk, Cesar de la Fuente-Nunez, Farren J Isaacs, Xuanhe Zhao, and Timothy K Lu. 2021. “Hydrogel-Based Biocontainment of Bacteria for Continuous Sensing and Computation.” Nature Chemical Biology 17 (6): 724–31.en_US
dc.identifier.issn1552-4450en_US
dc.identifier.issn1552-4469en_US
dc.identifier.urihttps://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37374324*
dc.description.abstractGenetically modified microorganisms (GMMs) can enable a wide range of important applications, including environmental sensing, precision therapeutics, and responsive materials. However, containment of GMMs to prevent environmental escape and satisfy regulatory requirements is a bottleneck for real-world use. While biochemical strategies have been developed to restrict unwanted growth and replication of GMMs in the environment, there is a need for deployable physical containment technologies to achieve redundant, multi-layered, and robust containment. In addition, form factors that enable easy retrieval would be useful for environmental sensing. To address this challenge, we developed a hydrogel-based encapsulation system for GMMs that incorporates a biocompatible multilayer tough shell and an alginate-based core. This DEployable Physical COntainment Strategy (DEPCOS) allows no detectable GMM escape, bacteria to be protected against environmental insults including antibiotics and low pH, controllable lifespan, and easy retrieval of genetically recoded bacteria. To highlight the versatility of a DEPCOS, we demonstrate that robustly encapsulated cells can execute useful functions, including performing cell-cell communication with other encapsulated bacteria and sensing heavy metals in water samples from the Charles River. We envision that our multilayered physical and chemical containment strategy will facilitate the realization of a wide range of real-world applications for "living" biosensors.en_US
dc.language.isoen_USen_US
dc.publisherSpringer Science and Business Media LLCen_US
dc.relationNature Chemical Biologyen_US
dc.relation.isversionofhttps://doi.org/10.1038/s41589-021-00779-6en_US
dash.licensePass Through
dc.subjectCell Biologyen_US
dc.subjectMolecular Biologyen_US
dc.titleHydrogel-Based Biocontainment of Bacteria for Continuous Sensing and Computationen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalNature Chemical Biologyen_US
dash.waiver2021-01-25
dc.date.available2023-02-10T14:15:43Z
dash.affiliation.otherHarvard Medical Schoolen_US
dc.identifier.doi10.1038/s41589-021-00779-6
dash.waiver.reasonRequested by the publisheren_US
dash.source.volume17en_US
dash.source.page724-731en_US
dash.source.issue6en_US
dash.contributor.affiliatedTang, Tzu-Chieh
dash.contributor.affiliatedIsaacs, Farren
dash.contributor.affiliatedZhao, Xuanhe
dash.contributor.affiliatedRovner, Alexis
dash.contributor.affiliatedLiu, Xinyue
dash.contributor.affiliatedYuk, Hyunwoo
dash.contributor.affiliatedLu, Timothy


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