Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor
View/ Open
s41587-019-0331-8.pdf (3.173Mb)
Access Status
Full text of the requested work is not available in DASH at this time ("restricted access"). For more information on restricted deposits, see our FAQ.Author
Lesman, Daniel
Published Version
https://doi.org/10.1038/s41587-019-0331-8Metadata
Show full item recordCitation
Chen, Haiqi, Sophia Liu, Samuel Padula, Daniel Lesman, Kettner Griswold, Allen Lin, Tongtong Zhao, Jamie L. Marshall, and Fei Chen. 2020. “Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor.” Nature Biotechnology 38 (2): 165–68.Abstract
Here, we describe TRACE, a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. Using TRACE in a MEK1 inhibitor resistance screen, we identified functionally correlated mutations.Citable link to this page
https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37374385
Collections
- FAS Scholarly Articles [18176]
Contact administrator regarding this item (to report mistakes or request changes)