Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor

Chen, Haiqi; Liu, Sophia; Padula, Samuel; Lesman, Daniel; Griswold, Kettner; Lin, Allen; Zhao, Tongtong; Marshall, Jamie; Chen, Fei

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Author

Chen, Haiqi HARVARD

Liu, Sophia HARVARD

Padula, Samuel HARVARD

Lesman, Daniel

Griswold, Kettner HARVARD

Lin, Allen HARVARD

Zhao, Tongtong HARVARD

Marshall, Jamie HARVARD

Chen, Fei

Published Version

https://doi.org/10.1038/s41587-019-0331-8

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Citation

Chen, Haiqi, Sophia Liu, Samuel Padula, Daniel Lesman, Kettner Griswold, Allen Lin, Tongtong Zhao, Jamie L. Marshall, and Fei Chen. 2020. “Efficient, Continuous Mutagenesis in Human Cells Using a Pseudo-Random DNA Editor.” Nature Biotechnology 38 (2): 165–68.

Abstract

Here, we describe TRACE, a method that enables continuous, targeted mutagenesis in human cells using a cytidine deaminase fused to T7 RNA polymerase. TRACE induces high rates of mutagenesis over multiple cell generations in genes under the control of a T7 promoter integrated in the genome. Using TRACE in a MEK1 inhibitor resistance screen, we identified functionally correlated mutations.

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https://nrs.harvard.edu/URN-3:HUL.INSTREPOS:37374385

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