Programming molecular behavior: development and applications of autonomous DNA synthesis cascades
dc.contributor.advisor | Shih, William | |
dc.contributor.author | Kishi, Jocelyn Yoshiko | |
dc.date.accessioned | 2019-05-16T12:41:16Z | |
dc.date.created | 2018-11 | |
dc.date.issued | 2018-09-25 | |
dc.date.submitted | 2018 | |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:39947207 | * |
dc.description.abstract | DNA serves many functional roles in living organisms, and dynamic DNA nanotechnology seeks to expand this set of DNA-based behaviors through the rational design of new functions. In the past 20 years, the predictable and programmable structure of DNA based on Watson-Crick base pairing has been utilized to engineer DNA-based chemical reaction pathways capable of performing a number of different tasks, including biosensing, molecular transport of cargo, and logic computation. In this dissertation, I present my work, in collaboration with others, to develop a new mechanism for engineering DNA-based molecular behaviors. The method, primer exchange reactions (PERs), can be used to autonomously synthesize single-stranded DNA using a set of synthetic catalytic hairpin oligos for a range of applications. After first describing the concept and implementation of the method, I go on to demonstrate several behaviors, including molecular sensing, recording, and actuation. I follow up with another application of PER for visualizing nucleic acids in situ, which can be used to reveal information about their spatial organization as well as function. Finally, I conclude with a discussion on the relevance of PER to the emerging field of molecular robotics. | |
dc.description.sponsorship | Systems Biology | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en | |
dash.license | LAA | |
dc.subject | Biology, Molecular | |
dc.subject | Biology, Genetics | |
dc.title | Programming molecular behavior: development and applications of autonomous DNA synthesis cascades | |
dc.type | Thesis or Dissertation | |
dash.depositing.author | Kishi, Jocelyn Yoshiko | |
dc.date.available | 2019-05-16T12:41:16Z | |
thesis.degree.date | 2018 | |
thesis.degree.grantor | Graduate School of Arts & Sciences | |
thesis.degree.level | Doctoral | |
thesis.degree.name | Doctor of Philosophy | |
dc.contributor.committeeMember | Wong, Wesley | |
dc.contributor.committeeMember | Hormoz, Sahand | |
dc.type.material | text | |
thesis.degree.department | Systems Biology | |
dash.identifier.vireo | http://etds.lib.harvard.edu/gsas/admin/view/2512 | |
dc.description.keywords | DNA computing; molecular robotics; molecular programming; DNA nanotechnology; DNA synthesis; DNA FISH; RNA FISH | |
dc.identifier.orcid | 0000-0003-4352-1159 | |
dash.author.email | jykishi@gmail.com |
Files in this item
This item appears in the following Collection(s)
-
FAS Theses and Dissertations [6902]