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dc.contributor.advisorShih, William
dc.contributor.authorKishi, Jocelyn Yoshiko
dc.date.accessioned2019-05-16T12:41:16Z
dc.date.created2018-11
dc.date.issued2018-09-25
dc.date.submitted2018
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:39947207*
dc.description.abstractDNA serves many functional roles in living organisms, and dynamic DNA nanotechnology seeks to expand this set of DNA-based behaviors through the rational design of new functions. In the past 20 years, the predictable and programmable structure of DNA based on Watson-Crick base pairing has been utilized to engineer DNA-based chemical reaction pathways capable of performing a number of different tasks, including biosensing, molecular transport of cargo, and logic computation. In this dissertation, I present my work, in collaboration with others, to develop a new mechanism for engineering DNA-based molecular behaviors. The method, primer exchange reactions (PERs), can be used to autonomously synthesize single-stranded DNA using a set of synthetic catalytic hairpin oligos for a range of applications. After first describing the concept and implementation of the method, I go on to demonstrate several behaviors, including molecular sensing, recording, and actuation. I follow up with another application of PER for visualizing nucleic acids in situ, which can be used to reveal information about their spatial organization as well as function. Finally, I conclude with a discussion on the relevance of PER to the emerging field of molecular robotics.
dc.description.sponsorshipSystems Biology
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectBiology, Molecular
dc.subjectBiology, Genetics
dc.titleProgramming molecular behavior: development and applications of autonomous DNA synthesis cascades
dc.typeThesis or Dissertation
dash.depositing.authorKishi, Jocelyn Yoshiko
dc.date.available2019-05-16T12:41:16Z
thesis.degree.date2018
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
dc.contributor.committeeMemberWong, Wesley
dc.contributor.committeeMemberHormoz, Sahand
dc.type.materialtext
thesis.degree.departmentSystems Biology
dash.identifier.vireohttp://etds.lib.harvard.edu/gsas/admin/view/2512
dc.description.keywordsDNA computing; molecular robotics; molecular programming; DNA nanotechnology; DNA synthesis; DNA FISH; RNA FISH
dc.identifier.orcid0000-0003-4352-1159
dash.author.emailjykishi@gmail.com


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