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dc.contributor.advisorSpringer, Michael
dc.contributor.authorGroff, Abigail F.
dc.date.accessioned2019-05-20T12:22:04Z
dc.date.created2018-05
dc.date.issued2018-04-18
dc.date.submitted2018
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:40050000*
dc.description.abstractWe currently understand very little about how the genome functions. Up to 80% of the genome is transcribed, but much of biology focuses on the ~1-5% that codes for proteins. This narrow focus leaves many newly cataloged noncoding transcripts wholly uncharacterized. For the noncoding transcripts that have been studied using cellular and molecular biology techniques, only a handful of animal models have been generated. My PhD work centers on studying the physiological relevance of a set of long intergenic noncoding RNAs (lincRNAs) implicated in development or disease. Specifically, my studies focus on demonstrating how these lincRNA loci affect gene regulation in their local gene neighborhoods (in cis). The first step in this quest is to disentangle whether the RNA transcript or the noncoding DNA within the lincRNA locus is responsible for genetic regulation. I have found that at least six of the thirteen lincRNA loci we examined may function in cis, and have demonstrated that at least two of these loci contain DNA-based enhancers that can explain the functionality of the locus. Collectively, these data provide a foundation from which to address the questions of how noncoding loci influence expression in their local gene neighborhoods, and which molecular species (DNA or RNA) is/are responsible for affecting that function.
dc.description.sponsorshipSystems Biology
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectBiology, Molecular
dc.subjectBiology, Bioinformatics
dc.subjectBiology, Cell
dc.titleFinding Cis-Regulatory Regions in Long Noncoding RNA Loci
dc.typeThesis or Dissertation
dash.depositing.authorGroff, Abigail F.
dc.date.available2019-05-20T12:22:04Z
thesis.degree.date2018
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
dc.contributor.committeeMemberMurray, Andrew
dc.contributor.committeeMemberMango, Susan
dc.type.materialtext
thesis.degree.departmentSystems Biology
dash.identifier.vireohttp://etds.lib.harvard.edu/gsas/admin/view/2035
dc.description.keywordslincRNA, cis regulation, regulatory DNA, lncRNA
dc.identifier.orcid0000-0002-7424-9410
dash.author.emailabbie.groff@gmail.com


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