Quantifying and Modeling Dynamics of Heat Shock Detection and Response in the Intestine of Caenorhabditis Elegans
Author
Dahlstrom, Erin Katrina
Metadata
Show full item recordAbstract
The heat shock response is the organized molecular response to stressors which disrupt proteostasis, potentially leading to protein misfolding and aggregation. While the regulation of the heat shock response is well-studied in single cells, its coordination at the cell, tissue, and systemic levels of a multicellular organism is more poorly understood. To probe the interplay between systemic and cell-autonomous responses, I studied the upregulation of HSP-16.2, a molecular chaperone induced throughout the intestine of Caenorhabditis elegans following a heat shock, by taking longitudinal measurements in a microfluidic environment. Based on the dynamics of HSP-16.2 accumulation, I showed that a combination of heat shock temperature and duration defines the intensity of stress inflicted on the worm and identified two regimes of low and high intensity. Modeling the underlying regulatory dynamics implicated the saturation of HSP mRNA production in defining these two regimes and emphasized the importance of time separation between transcription and translation in establishing these dynamics. By applying heat shock and measuring response in separate parts of the animals, I implicated thermosensory neurons in accelerating the response and transducing information within the animal. I discuss possible implications of the systemic and cell level aspects and how they coordinate to facilitate the organismal response.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:40050014
Collections
- FAS Theses and Dissertations [6136]
Contact administrator regarding this item (to report mistakes or request changes)