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dc.contributor.advisorMurray, Andrew
dc.contributor.advisorGibbs, Karine A.
dc.contributor.authorLittle, Kristin
dc.date.accessioned2019-08-09T09:26:39Z
dash.embargo.terms2019-05-01
dc.date.created2017-05
dc.date.issued2017-05-08
dc.date.submitted2017
dc.identifier.citationLittle, Kristin. 2017. Outer Membrane Structures Regulate Proteus Mirabilis Swarm Motility. Doctoral dissertation, Harvard University, Graduate School of Arts & Sciences.
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41142068*
dc.description.abstractThe Gram negative bacterium Proteus mirabilis engages in a social, surface-based motility called swarming. Swarming requires two broad phenomena: swarmer cell development and population migration. The genetic requirements for initiation of swarmer cell development have been well characterized; however, the differential requirements for swarmer cell development and swarmer cell motility remain poorly understood. Here we characterize the roles of two sugar-based outer membrane structures in regulating P. mirabilis swarming. First, we demonstrate that cells require the poorly understood Enterobacterial Common Antigen (ECA) for maintenance of cell envelope integrity during swarmer cell development; this functions occurs in part through a cell envelope stress-sensing pathways. Next, we demonstrate that cells require lipopolysaccharide O-antigen for population expansion. We demonstrate that agar concentration (a proxy for surface wettability) modulates the behavior of swarming cells at the micron and centimeter scale, and propose that O-antigen enhances swarming through enhanced wettability of the local surface environment. Altogether, this research demonstrates that two seemingly similar sugar-based molecules (ECA and O-antigen) play different roles at different scales in promoting P. mirabilis swarm motility. These molecules are virulence-associated and shared across many species of bacteria, including known pathogens. As such, this work may have broad implications for bacterial behavior and virulence.
dc.description.sponsorshipBiology, Molecular and Cellular
dc.format.mimetypeapplication/pdf
dc.language.isoen
dash.licenseLAA
dc.subjectProteus mirabilis
dc.subjectswarm motility
dc.subjectcell development
dc.subjectouter membrane
dc.titleOuter Membrane Structures Regulate Proteus Mirabilis Swarm Motility
dc.typeThesis or Dissertation
dash.depositing.authorLittle, Kristin
dash.embargo.until2019-05-01
dc.date.available2019-08-09T09:26:39Z
thesis.degree.date2017
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.grantorGraduate School of Arts & Sciences
thesis.degree.levelDoctoral
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy
thesis.degree.nameDoctor of Philosophy
dc.contributor.committeeMemberBalskus, Emily
dc.contributor.committeeMemberGarner, Ethan
dc.type.materialtext
thesis.degree.departmentBiology, Molecular and Cellular
thesis.degree.departmentBiology, Molecular and Cellular
dash.identifier.vireo
dc.identifier.orcid0000-0002-2522-4233
dash.author.emailkristin.little87@gmail.com


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