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dc.contributor.authorLee, Sungon
dc.contributor.authorVinegoni, Claudio
dc.contributor.authorFeruglio, Paolo Fumene
dc.contributor.authorWeissleder, Ralph
dc.date.accessioned2019-09-21T03:35:18Z
dc.date.issued2012
dc.identifier.citationLee, Sungon, Claudio Vinegoni, Paolo Fumene Feruglio, and Ralph Weissleder. 2012. “Improved Intravital Microscopy via Synchronization of Respiration and Holder Stabilization.” Journal of Biomedical Optics 17 (9): 960181. https://doi.org/10.1117/1.jbo.17.9.096018.
dc.identifier.issn1083-3668
dc.identifier.issn1560-2281
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384190*
dc.description.abstractA major challenge in high-resolution intravital confocal and multiphoton microscopy is physiologic tissue movement during image acquisition. Of the various physiological sources of movement, respiration has arguably the largest and most wide-ranging effect. We describe a technique for achieving stabilized microscopy imaging using a dual strategy. First, we designed a mechanical stabilizer for constraining physical motion; this served to simultaneously increase the in-focus range over which data can be acquired as well as increase the reproducibility of imaging a certain position within each confocal imaging plane. Second, by implementing a retrospective breathing-gated imaging modality, we performed selective image extraction gated to a particular phase of the respiratory cycle. Thanks to the high reproducibility in position, all gated images presented a high degree of correlation over time. The images obtained using this technique not only showed significant improvements over images acquired without the stabilizer, but also demonstrated accurate in vivo imaging during longitudinal studies. The described methodology is easy to implement with any commercial imaging system, as are used by most biological imaging laboratories, and can be used for both confocal and multiphoton laser scanning microscopy. c 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.9.096018]
dc.language.isoen_US
dc.publisherSociety of Photo-optical Instrumentation Engineers
dash.licenseLAA
dc.titleImproved intravital microscopy via synchronization of respiration and holder stabilization
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalJournal of Biomedical Optics
dash.depositing.authorWeissleder, Ralph::ea07ce19f187d4fab47c56ee97fa5c5a::600
dc.date.available2019-09-21T03:35:18Z
dash.workflow.comments1Science Serial ID 50473
dc.identifier.doi10.1117/1.JBO.17.9.096018
dash.source.volume17;9


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