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dc.contributor.authorMorton, Angela M.
dc.contributor.authorSefik, Esen
dc.contributor.authorUpadhyay, Rabi
dc.contributor.authorWeissleder, Ralph
dc.contributor.authorBenoist, Christophe
dc.contributor.authorMathis, Diane
dc.date.accessioned2019-09-21T03:35:38Z
dc.date.issued2014
dc.identifier.citationMorton, A. M., E. Sefik, R. Upadhyay, R. Weissleder, C. Benoist, and D. Mathis. 2014. “Endoscopic Photoconversion Reveals Unexpectedly Broad Leukocyte Trafficking to and from the Gut.” Proceedings of the National Academy of Sciences 111 (18): 6696–6701. https://doi.org/10.1073/pnas.1405634111.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384222*
dc.description.abstractGiven mounting evidence of the importance of gut-microbiota/immune-cell interactions in immune homeostasis and responsiveness, surprisingly little is known about leukocyte movements to, and especially from, the gut. We address this topic in a minimally perturbant manner using Kaede transgenic mice, which universally express a photoconvertible fluorescent reporter. Transcutaneous exposure of the cervical lymph nodes to violet light permitted punctual tagging of immune cells specifically therein, and subsequent monitoring of their immigration to the intestine; endoscopic flashing of the descending colon allowed specific labeling of intestinal leukocytes and tracking of their emigration. Our data reveal an unexpectedly broad movement of leukocyte subsets to and from the gut at steady state, encompassing all lymphoid and myeloid populations examined. Nonetheless, different subsets showed different trafficking proclivities (e. g., regulatory T cells were more restrained than conventional T cells in their exodus from the cervical lymph nodes). The novel endoscopic approach enabled us to evidence gut-derived Th17 cells in the spleens of K/BxN mice at the onset of their genetically determined arthritis, thereby furnishing a critical mechanistic link between the intestinal microbiota, namely segmented filamentous bacteria, and an extraintestinal autoinflammatory disease.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleEndoscopic photoconversion reveals unexpectedly broad leukocyte trafficking to and from the gut
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorWeissleder, Ralph::ea07ce19f187d4fab47c56ee97fa5c5a::600
dc.date.available2019-09-21T03:35:38Z
dash.workflow.comments1Science Serial ID 91136
dc.identifier.doi10.1073/pnas.1405634111
dash.source.volume111;18
dash.source.page6696


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