A single reporter for targeted multimodal in vivo imaging
View/ Open
Author
Niers, Johanna
Chen, John
Lewandrowski, Grant
Kerami, Mariam
Garanger, Elisabeth
Wojtkiewicz, Greg
Waterman, Peter
Keliher, Edmund
Weissleder, Ralph
Tannous, Bakhos
Published Version
https://doi.org/10.1021/ja209868gMetadata
Show full item recordCitation
Niers, Johanna M, John W Chen, Grant Lewandrowski, Mariam Kerami, Elisabeth Garanger, Greg Wojtkiewicz, Peter Waterman, Edmund Keliher, Ralph Weissleder, and Bakhos A. Tannous. 2012. “Single Reporter for Targeted Multimodal in Vivo Imaging.” Journal of the American Chemical Society 134 (11): 5149–56. https://doi.org/10.1021/ja209868g.Abstract
We have developed a multifaceted, highly specific reporter for multimodal in vivo imaging and applied it for detection of brain tumors. A metabolically biotinylated, membrane-bound form of Gaussia luciferase was synthesized, termed mbGluc-biotin. We engineered glioma cells to express this reporter and showed that brain tumor formation can be temporally imaged by bioluminescence following systemic administration of coelenterazine. Brain tumors expressing this reporter had high sensitivity for detection by magnetic resonance and fluorescence tomographic imaging upon injection of streptavidin conjugated to magnetic nanoparticles or fluorophore, respectively. Moreover, single photon emission computed tomography showed enhanced imaging of these tumors upon injection with streptavidin complexed to wIn-DTPA-biotin. This work shows for the first time a single small reporter (similar to 40 kDa) which can be monitored with most available molecular imaging modalities and can be extended for single cell imaging using intravital microscopy, allowing real-time tracking of any cell expressing it in vivo.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:41384231
Collections
- HMS Scholarly Articles [17875]
Contact administrator regarding this item (to report mistakes or request changes)