Fluorescence polarization-based nucleic acid testing for rapid and cost-effective diagnosis of infectious disease
Park, Ki Soo
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CitationPark, Ki Soo, Richelle C. Charles, Edward T. Ryan, Ralph Weissleder, and Hakho Lee. 2015. “Fluorescence Polarization Based Nucleic Acid Testing for Rapid and Cost-Effective Diagnosis of Infectious Disease.” Chemistry - A European Journal 21 (46): 16359–63. https://doi.org/10.1002/chem.201502934.
AbstractA new nucleic acid detection method was developed for a rapid and cost-effective diagnosis of infectious disease. This approach relies on the three unique elements: 1)detection probes that regulate DNA polymerase activity in response to the complementary target DNA; 2)universal reporters conjugated with a single fluorophore; and 3)fluorescence polarization (FP) detection. As a proof-of-concept, the assay was used to detect and sub-type Salmonella bacteria with sensitivities down to a single bacterium in less than three hours.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384238
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