A secreted luciferase for ex-vivo monitoring of in vivo processes

 
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Wurdinger, Thomas
Badr, Christian
Pike, Lisa
Kleine, Ruben de
Weissleder, Ralph
Breakefield, Xandra O.
Tannous, Bakhos A.
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https://doi.org/10.1038/nmeth.1177
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Wurdinger, Thomas, Christian Badr, Lisa Pike, Ruben de Kleine, Ralph Weissleder, Xandra O Breakefield, and Bakhos A Tannous. 2008. “A Secreted Luciferase for Ex Vivo Monitoring of in Vivo Processes.” Nature Methods 5 (2): 171–73. https://doi.org/10.1038/nmeth.1177.
Abstract
Luciferases are widely used to monitor biological processes. Here we describe the naturally secreted Gaussia princeps luciferase (Gluc) as a highly sensitive reporter for quantitative assessment of cells in vivo by measuring its concentration in blood. The Gluc blood assay complements in vivo bioluminescence imaging, which has the ability to localize the signal and provides a multifaceted assessment of cell viability, proliferation and location in experimental disease and therapy models.
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:41384309

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